AXL Monoclonal antibody, PBS Only

• 

  WB analysis using 68108-1-Ig (same clone as 68108-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68108-1-Ig (AXL antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68108-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 68108-1-Ig (same clone as 68108-1-PBS)

  WB result of AXL antibody (68108-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AXL transfected HeLa cells. This data was developed using the same antibody clone with 68108-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

AXL Monoclonal Antibody for WB, Indirect ELISA

Cat No. 68108-1-PBS

Clone No.3D12F11

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

3D12F11, EC:2.7.10.1, Tyrosine-protein kinase receptor UFO, UFO

---

Product Information

68108-1-PBS targets AXL in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14401

  Product name: Recombinant human AXL protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 545-894 aa of BC032229

  Sequence: GEFGAVMEGQLNQDDSILKVAVKTMKIAICTRSELEDFLSEAVCMKEFDHPNVMRLIGVCFQGSERESFPAPVVILPFMKHGDLHSFLLYSRLGDQPVYLPTQMLVKFMADIASGMEYLSTKRFIHRDLAARNCMLNENMSVCVADFGLSKKIYNGDYYRQGRIAKMPVKWIAIESLADRVYTSKSDVWSFGVTMWEIATRGQTPYPGVENSEIYDYLRRGNRLKQPADCLDGLYALMSRCWELNPQDRPSFTELREDLENTLKALPPAQEPDEILYVNMDEGGGYPEPPGAAGGADPPTQPDPKDSCSCLTAAEVHPAGRYVLCPSTTPSPAQPADRGSPAAPGQEDGA

• Full Name: AXL receptor tyrosine kinase
• Calculated Molecular Weight: 894 aa, 98 kDa
• Observed Molecular Weight: 130-140 kDa
• GenBank Accession Number: BC032229
• Gene Symbol: AXL
• Gene ID (NCBI): 558
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P30530
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Axl, also known as Ufo or Ark, is a receptor tyrosine kinase of the TAM (Tyro3, Axl and Mer) family. Axl is a single-pass type I membrane protein containing two fibronectin type-III domains, two Ig-like C2-type domains and one protein kinase domain. Axl mRNA is widely expressed with highest levels in the heart and skeletal muscle. Gas6 has been identified as a ligand for human Axl. Gas6/Axl signaling plays a role in cell survival, cell proliferation, migration and differentiation. It has been reported that 110-120 kDa non-glycosylated AXL protein can be detected(PMID: 31171001). Endogenous Axl is auto-phosphorylated and reacted with a tyrosine-phosphorylated protein of 140 kDa(PMID: 12730092).