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PSMA8 Monoclonal antibody

PSMA8 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 68123-1-Ig

Clone No. 2G2G3

Publications (1)

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Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig, rabbit and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PSMA7L, PSMA8

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

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Filter: AllWBIHCIF/ICC

WB analysis using 68123-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 68123-1-Ig (PSMA8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of rat lung using 68123-1-Ig
rat lung tissue were subjected to SDS PAGE followed by western blot with 68123-1-Ig (PSMA8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse lung using 68123-1-Ig
mouse lung tissue were subjected to SDS PAGE followed by western blot with 68123-1-Ig (PSMA8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 68123-1-Ig
WB result of PSMA8 antibody (68123-1-Ig; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMA8 transfected HeLa cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 68123-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68123-1-Ig (PSMA8 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse liver using 68123-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68123-1-Ig (PSMA8 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat liver using 68123-1-Ig
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 68123-1-Ig (PSMA8 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat testis using 68123-1-Ig
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 68123-1-Ig (PSMA8 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung cancer using 68123-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68123-1-Ig (PSMA8 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse testis using 68123-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68123-1-Ig (PSMA8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse testis using 68123-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68123-1-Ig (PSMA8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 68123-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSMA8 antibody (68123-1-Ig, Clone: 2G2G3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for PSMA8 in ?

Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HCT 116 cells, rat lung tissue, mouse lung tissue, HeLa cells, HuH-7 cells, NCI-H1299 cells, A549 cells, pig lung tissue, rabbit lung tissue
Positive IHC detected in	mouse testis tissue, human colon cancer tissue, human lung cancer tissue, mouse liver tissue, rat liver tissue, rat testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68123-1-Ig targets PSMA8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Reactivity	Human, mouse, rat, pig, rabbit
Cited Reactivity	human
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag5138 Product name: Recombinant human PSMA8 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-250 aa of BC042820 Sequence: MASRYDRAITVFSPDGHLFQVEYAQEAVKKGSTAVGIRGTNIVVLGVEKKSVAKLQDERTVRKICALDDHVCMAFAGLTADARVVINRARVECQSHKLTVEDPVTVEYITRFIATLKQKYTQSNGRRPFGISALIVGFDDDGISRLYQTDPSGTYHAWKANAIGRSAKTVREFLEKNYTEDAIASDSEAIKLAIKALLEVVQSGGKNIELAIIRRNQPLKMFSAKEVELYVTEIEKEKEEAEKKKSKKSV Predict reactive species
Full Name	proteasome (prosome, macropain) subunit, alpha type, 8
Calculated Molecular Weight	29 kDa
Observed Molecular Weight	28 kDa
GenBank Accession Number	BC042820
Gene Symbol	PSMA8
Gene ID (NCBI)	143471
RRID	AB_2923651
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q8TAA3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protocols

Product Specific Protocols
IF protocol for PSMA8 antibody 68123-1-Ig	Download protocol
IHC protocol for PSMA8 antibody 68123-1-Ig	Download protocol
WB protocol for PSMA8 antibody 68123-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The PSMA8 Monoclonal antibody (2G2G3), catalog number 68123-1-Ig, has 1 total citation. It was cited in Molecular Cancer (Impact Factor: 42.2), in a 2025 study investigating therapy-induced senescence as a transient drug resistance mechanism in breast cancer. The antibody was used for Western Blot (WB) on human samples, placing its cited research field in oncology, specifically breast cancer biology and treatment resistance mechanisms.