Tested Applications
| Positive WB detected in | FITC-TDP43, FITC-Ag1231, BSA |
| Positive ELISA detected in | FITC conjugated antibody |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Enzyme-linked Immunosorbent Assay (ELISA) | ELISA : |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68132-1-Ig targets FITC in WB, ELISA applications and shows reactivity with chemical compound, fitc samples.
| Tested Reactivity | chemical compound, fitc |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | FITC |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2923659 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications. It is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. It is typically available as a mixture of isomers, fluorescein 5-isothiocyanate (5-FITC) and fluorescein 6-isothiocyanate (6-FITC). FITC is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. CAS number of FITC: 27072-45-3 (mixture); 3326-32-7, 5-isomer; 18861-78-4 (6-isomer). This mouse monoclonal antibody detects the FITC only. This antibody can be coated in the plate to capture the FITC conjugated protein or antibody.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FITC antibody 68132-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FITC Monoclonal antibody (3C10A1), catalog number 68132-1-Ig, has 2 total citations. These appear in Advanced Science and Environmental Health Perspectives. The cited research spans antiviral molecular design targeting enteroviral RNAi suppressors and environmental toxicology examining organophosphorus flame retardants' effects on adiponectin receptors and metabolic disruption. Both studies utilized the antibody for Western blot applications.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Rational Design of Broad-Spectrum Anti-Enteroviral Molecular Glues Targeting Enteroviral RNAi Suppressors. | ||
Environ Health Perspect Organophosphorus Flame Retardants and Metabolic Disruption: An in Silico, in Vitro, and in Vivo Study Focusing on Adiponectin Receptors |
