Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, HEK-293 cells, K-562 cells, pig adrenal gland tissue, human testis tissue, rat testis tissue, mouse testis tissue |
| Positive IF-P detected in | mouse cerebellum tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68181-1-Ig targets COMT in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6502 Product name: Recombinant human COMT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-182 aa of BC000419 Sequence: MNVGDKKGKIVDAVIQEHQPSVLLELGAYCGYSAVRMARLLSPGARLITIEINPDCAAITQRMVDFAGMKDKVTLVVGASQDIIPQLKKKYDVDTLDMVFLDHWKDRYLPDTLLLEECGLLRKGTVLLADNVICPGAPDFLAHVRGSSCFECTHYQSFLEYREVVDGLEKAIYKGPGSEAGP Predict reactive species |
| Full Name | catechol-O-methyltransferase |
| Calculated Molecular Weight | 30 kDa |
| Observed Molecular Weight | 28-30 kDa |
| GenBank Accession Number | BC000419 |
| Gene Symbol | COMT |
| Gene ID (NCBI) | 1312 |
| RRID | AB_2935272 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P21964 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Catechol O-methyltransferase (COMT) is an important enzyme that catalyzes O-methylation and inactivation of metabolically active catechol-containing bioactive molecules and drugs. The mammalian COMT enzyme exists in soluble form (24-26 kDa) and membrane-bound form (28-30 kDa), which are encoded by a single gene utilizing different promoters.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for COMT antibody 68181-1-Ig | Download protocol |
| WB protocol for COMT antibody 68181-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The COMT Monoclonal antibody (1A1A4), catalog number 68181-1-Ig, has a total of 1 citation. This citation appears in Experimental Eye Research (Impact Factor 3.1), published in 2026. The study, titled "Proteomic profiling of human corneal stroma reveals molecular signatures associated with myopia progression," falls within the fields of ophthalmology and proteomics, specifically investigating molecular mechanisms underlying myopia.
| Species | Application | Title |
|---|---|---|
Exp Eye Res Proteomic profiling of human corneal stroma reveals molecular signatures associated with myopia progression. |
