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  • Western Blot (WB) analysis of various lysates using SNX12 Monoclonal antibody (68260-1-Ig)

    WB analysis using 68260-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68260-1-Ig (SNX12 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SNX12 Monoclonal antibody (68260-1-Ig)

    IF Staining of HeLa using 68260-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SNX12 antibody (68260-1-Ig, Clone: 2C6C8 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red).

SNX12 Monoclonal antibody

SNX12 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68260-1-Ig
Clone No.2C6C8

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Sorting nexin-12, 2C6C8

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inA549 cells, MCF-7 cells, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68260-1-Ig targets SNX12 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SNX12 fusion protein Ag3014 Predict reactive species
Full Name sorting nexin 12
Calculated Molecular Weight 172 aa, 20 kDa
Observed Molecular Weight18 kDa
GenBank Accession NumberBC020559
Gene Symbol SNX12
Gene ID (NCBI) 29934
RRIDAB_2935345
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UMY4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX12 (Sorting nexin 12) is localized in early endosomes and SNX12 overexpression specifically prevents the degradative pathway from early to late endosomes/lysosomes (PMID: 22719997). SNX12 may participate in Alzheimer's disease (AD) pathology by regulating the endocytosis of BACE1 and affecting β-processing of APP (PMID: 22709416).

Protocols

Product Specific Protocols
WB protocol for SNX12 antibody 68260-1-IgDownload protocol
IF protocol for SNX12 antibody 68260-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols