SNX12 Monoclonal antibody, PBS Only

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  WB analysis using 68260-1-Ig (same clone as 68260-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68260-1-Ig (SNX12 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68260-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68260-1-Ig (same clone as 68260-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SNX12 antibody (68260-1-Ig, Clone: 2C6C8 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 68260-1-PBS in a different storage buffer formulation.

SNX12 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 68260-1-PBS

Clone No.2C6C8

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Sorting nexin-12, 2C6C8

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Product Information

68260-1-PBS targets SNX12 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: SNX12 fusion protein Ag3014
• Full Name: sorting nexin 12
• Calculated Molecular Weight: 172 aa, 20 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC020559
• Gene Symbol: SNX12
• Gene ID (NCBI): 29934
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UMY4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX12 (Sorting nexin 12) is localized in early endosomes and SNX12 overexpression specifically prevents the degradative pathway from early to late endosomes/lysosomes (PMID: 22719997). SNX12 may participate in Alzheimer's disease (AD) pathology by regulating the endocytosis of BACE1 and affecting β-processing of APP (PMID: 22709416).