Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells, HepG2 cells, HEK-293 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:750-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 2 publications below |
Product Information
68270-1-Ig targets RPS20 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8275 Product name: Recombinant human RPS20 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-119 aa of BC007507 Sequence: MAFKDTGKTPVEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA Predict reactive species |
| Full Name | ribosomal protein S20 |
| Calculated Molecular Weight | 13 kDa |
| Observed Molecular Weight | 16 kDa |
| GenBank Accession Number | BC007507 |
| Gene Symbol | RPS20 |
| Gene ID (NCBI) | 6224 |
| RRID | AB_2935353 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P60866 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS20 is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RPS20 antibody 68270-1-Ig | Download protocol |
| IHC protocol for RPS20 antibody 68270-1-Ig | Download protocol |
| WB protocol for RPS20 antibody 68270-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Functional screen identifies RBM42 as a mediator of oncogenic mRNA translation specificity | ||
Sci Rep Hydrogen sulfide preserves intestinal barrier repair function through sulfhydration of RPS20 in experimental colitis | ||
