Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells, HepG2 cells, HEK-293 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:750-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68270-1-Ig targets RPS20 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8275 Product name: Recombinant human RPS20 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-119 aa of BC007507 Sequence: MAFKDTGKTPVEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA Predict reactive species |
| Full Name | ribosomal protein S20 |
| Calculated Molecular Weight | 13 kDa |
| Observed Molecular Weight | 16 kDa |
| GenBank Accession Number | BC007507 |
| Gene Symbol | RPS20 |
| Gene ID (NCBI) | 6224 |
| RRID | AB_2935353 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P60866 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS20 is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RPS20 antibody 68270-1-Ig | Download protocol |
| WB protocol for RPS20 antibody 68270-1-Ig | Download protocol |
| IF protocol for RPS20 antibody 68270-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RPS20 Monoclonal antibody (3E9B4), catalog number 68270-1-Ig, has 4 total citations. These appear in Nature Cell Biology, Life Sciences, Scientific Reports, and bioRxiv. The associated research fields include oncogenic mRNA translation specificity, inflammatory and oxidative stress signaling in sepsis, and intestinal barrier repair in colitis.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Functional screen identifies RBM42 as a mediator of oncogenic mRNA translation specificity | ||
Life Sci RPS20 phosphorylation acts as a molecular switch to integrate inflammatory and oxidative stress signals in sepsis. | ||
Sci Rep Hydrogen sulfide preserves intestinal barrier repair function through sulfhydration of RPS20 in experimental colitis | ||
