Filter: AllWBIHCIF/ICCCytometric bead array

WB analysis using 68270-1-Ig (same clone as 68270-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 68270-1-Ig (RPS20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68270-1-PBS in a different storage buffer formulation.
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Various lysates were subjected to SDS PAGE followed by western blot with 68270-1-Ig (RPS20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68270-1-PBS in a different storage buffer formulation.
WB analysis using 68270-1-Ig (same clone as 68270-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 68270-1-Ig (RPS20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68270-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 68270-1-Ig (same clone as 68270-1-PBS)
WB result of RPS20 antibody (68270-1-Ig; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPS20 transfected HepG2 cells. This data was developed using the same antibody clone with 68270-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 68270-1-Ig (same clone as 68270-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68270-1-Ig (RPS20 antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 68270-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 68270-1-Ig (same clone as 68270-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RPS20 antibody (68270-1-Ig, Clone: 3E9B4 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68270-1-PBS in a different storage buffer formulation.
Cytometric bead array standard curve of MP50774-1
Cytometric bead array standard curve of MP50774-1, RPS20 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68270-2-PBS. Detection antibody: 68270-1-PBS. Standard:Ag8275. Range: 1.563-200 ng/mL.
Cytometric bead array standard curve of MP50774-2
Cytometric bead array standard curve of MP50774-2, RPS20 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68270-3-PBS. Detection antibody: 68270-1-PBS. Standard:Ag8275. Range: 3.125-200 ng/mL.

Featured Product
KD/KO Validated

RPS20 Monoclonal antibody, PBS Only (Detector)

RPS20 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 68270-1-PBS

Clone No.3E9B4

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

3E9B4, 40S ribosomal protein S20, ribosomal protein S20, Small ribosomal subunit protein uS10

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

68270-1-PBS targets RPS20 as part of a matched antibody pair:

MP50774-1: 68270-2-PBS capture and 68270-1-PBS detection (validated in Cytometric bead array)

MP50774-2: 68270-3-PBS capture and 68270-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag8275 Product name: Recombinant human RPS20 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-119 aa of BC007507 Sequence: MAFKDTGKTPVEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA Predict reactive species
Full Name	ribosomal protein S20
Calculated Molecular Weight	13 kDa
Observed Molecular Weight	16 kDa
GenBank Accession Number	BC007507
Gene Symbol	RPS20
Gene ID (NCBI)	6224
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P60866
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS20 is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm.