Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68292-1-Ig targets SNX3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1160 Product name: Recombinant human SNX3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-162 aa of BC008444 Sequence: MAETVADTRRLITKPQNLNDAYGPPSNFLEIDVSNPQTVGVGRGRFTTYEIRVKTNLPIFKLKESTVRRRYSDFEWLRSELERESKVVVPPLPGKAFLRQLPFRGDDGIFDDNFIEERKQGLEQFINKVAGHPLAQNERCLHMFLQDEIIDKSYTPSKIRHA Predict reactive species |
| Full Name | sorting nexin 3 |
| Calculated Molecular Weight | 19 kDa |
| Observed Molecular Weight | 16-20 kDa |
| GenBank Accession Number | BC008444 |
| Gene Symbol | SNX3 |
| Gene ID (NCBI) | 8724 |
| RRID | AB_2935372 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O60493 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SNX3 is a member of the sorting nexin family of proteins regulating membrane traffic. SNX3 regulates endosomal function through its PX (phox homology)-domain-mediated interaction with PtdIns(3)P (PMID: 11433298). It is a potential regulator of neurite formation and may play an important role in the development and function of the central nervous system (PMID: 19576982; 20817026).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SNX3 antibody 68292-1-Ig | Download protocol |
| WB protocol for SNX3 antibody 68292-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The antibody performed well in Western Blot, producing a strong band that enabled the user to assess overexpression levels and siRNA knock-down efficiency in their cell lines. The reviewer rated it 5 stars, indicating high satisfaction with its performance and reliability for quantification purposes.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ioana (Verified Customer) (08-28-2025) | This antibody gave a good strong band in Western Blotting, allowing me to calculate the amount of overexpression and siRNA knock-down efficiency in my cell lines.
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