Tested Applications
| Positive WB detected in | HaCaT cells, A431 cells, human saliva, rat skin tissue, mouse skin tissue |
| Positive IHC detected in | human cervical cancer tissue, human oesophagus cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1150-1:4600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68294-1-Ig targets Cytokeratin 6A in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0882 Product name: Recombinant human KRT6A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 364-564 aa of BC008807 Sequence: QVTAGRHGDDLRNTKQEIAEINRMIQRLRSEIDHVKKQCANLQAAIADAEQRGEMALKDAKNKLEGLEDALQKAKQDLARLLKEYQELMNVKLALDVEIATYRKLLEGEECRLNGEGVGQVNISVVQSTVSSGYGGASGVGSGLGLGGGSSYSYGSGLGVGGGFSSSSGRAIGGGLSSVGGGSSTIKYTTTSSSSRKSYKH Predict reactive species |
| Full Name | keratin 6A |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC008807 |
| Gene Symbol | Cytokeratin 6A |
| Gene ID (NCBI) | 3853 |
| RRID | AB_2935374 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P02538 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 6 is a type II keratin. It is used as a marker for epidermal hyperproliferation and differentiation.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Cytokeratin 6A antibody 68294-1-Ig | Download protocol |
| WB protocol for Cytokeratin 6A antibody 68294-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Cytokeratin 6A Monoclonal antibody (1G1D7), catalog number 68294-1-Ig, has a total of 2 citations. These appear in Cancer Cell and Cell Host Microbe. The cited research spans cancer biology (lymphovascular invasion and lymphatic metastasis) and cell death mechanisms (ferroptosis). Both citations used the antibody for immunofluorescence (IF) applications in mouse and rat species, respectively.
| Species | Application | Title |
|---|---|---|
Cancer Cell PDGFRα+ITGA11+ fibroblasts foster early-stage cancer lymphovascular invasion and lymphatic metastasis via ITGA11-SELE interplay | ||
Cell Host Microbe Rothia mucilaginosa membrane vesicles stabilize labile iron to alleviate UVB-induced ferroptosis. |
