Tested Applications
| Positive WB detected in | HCT 116 cells, HAP1 cells, LNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | human liver cancer tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68303-1-Ig targets DLAT in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, duck |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4226 Product name: Recombinant human DLAT protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-322 aa of BC039084 Sequence: MWRVCARRAQNVAPWAGLEARWTALQEVPGTPRVTSRSGPAPARRNSVTTGYGGVRALCGWTPSSGATPRNRLLLQLLGSPGRRYYSLPPHQKVPLPSLSPTMQAGTIARWEKKEGDKINEGDLIAEVETDKATVGFESLEECYMAKILVAEGTRDVPIGAIICITVGKPEDIEAFKNYTLDSSAAPTPQAAPAPTPAATASPPTPSAQAPGSSYPPHMQVLLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEGYLAKILVPEGTRDVPLGTPLCIIVEKEADISAFADYRPTEVTDLKPQVPPPT Predict reactive species |
| Full Name | dihydrolipoamide S-acetyltransferase |
| Calculated Molecular Weight | 647 aa, 70 kDa |
| Observed Molecular Weight | 69-70 kDa |
| GenBank Accession Number | BC039084 |
| Gene Symbol | DLAT |
| Gene ID (NCBI) | 1737 |
| RRID | AB_2935382 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P10515 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DLAT antibody 68303-1-Ig | Download protocol |
| IHC protocol for DLAT antibody 68303-1-Ig | Download protocol |
| WB protocol for DLAT antibody 68303-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DLAT Monoclonal Antibody (1F2B9) (Cat# 68303-1-Ig) has 35 citations spanning journals including ACS Nano, Advanced Science, Cell Reports, Redox Biology, Journal of Hematology & Oncology, Free Radical Biology & Medicine, Cell Death & Disease, and Pharmacological Research. Research fields center on cuproptosis, ferroptosis, and programmed cell death, with applications in oncology (lung, gastric, colorectal, liver, prostate, glioblastoma), neurodegeneration (Parkinson's, Alzheimer's), cardiac injury, renal disease, and nanomedicine-based immunotherapy.
| Species | Application | Title |
|---|---|---|
J Hematol Oncol Glutathione reductase deficiency potentiates the immunogenicity of ferroptosis and cuproptosis via amplified reactive oxygen species accumulation and cGAS-STING pathway activation | ||
Int J Oral Sci Cuproptosis promotes inflammatory osteolysis via GYS1-mediated glycogen metabolism. | ||
ACS Nano Tumor-Directed Disulfidptosis via Spatiotemporally Controlled Copper Bioorthogonal Activation. | ||
ACS Nano UiO-66 MOFs-Based "Epi-Nano-Sonosensitizer" for Ultrasound-Driven Cascade Immunotherapy against B-Cell Lymphoma | ||
Redox Biol Iron overload exaggerates renal ischemia-reperfusion injury by promoting tubular cuproptosis via interrupting function of LIAS | ||
J Exp Clin Cancer Res o8G-modified circKIAA1797 promotes lung cancer development by inhibiting cuproptosis |
