DLAT Monoclonal antibody, PBS Only

• 

  WB analysis using 68303-1-Ig (same clone as 68303-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68303-1-Ig (DLAT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68303-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HAP1 using 68303-1-Ig (same clone as 68303-1-PBS)

  WB result of DLAT antibody (68303-1-Ig; 1:20000; room temperature for 1.5 hours) with wild-type and sg-DLAT transfected HAP1 cells. This data was developed using the same antibody clone with 68303-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 68303-1-Ig (same clone as 68303-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68303-1-Ig (DLAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68303-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 68303-1-Ig (same clone as 68303-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68303-1-Ig (DLAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68303-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 68303-1-Ig (same clone as 68303-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68303-1-Ig (DLAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68303-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 68303-1-Ig (same clone as 68303-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68303-1-Ig (DLAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68303-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 68303-1-Ig (same clone as 68303-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DLAT antibody (68303-1-Ig, Clone: 1F2B9 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68303-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 68303-1-Ig (same clone as 68303-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DLAT antibody (68303-1-Ig, Clone: 1F2B9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68303-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

DLAT Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 68303-1-PBS

Clone No.1F2B9

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

1F2B9, Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial, DLTA, EC:2.3.1.12, ODP2

---

Product Information

68303-1-PBS targets DLAT in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4226

  Product name: Recombinant human DLAT protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-322 aa of BC039084

  Sequence: MWRVCARRAQNVAPWAGLEARWTALQEVPGTPRVTSRSGPAPARRNSVTTGYGGVRALCGWTPSSGATPRNRLLLQLLGSPGRRYYSLPPHQKVPLPSLSPTMQAGTIARWEKKEGDKINEGDLIAEVETDKATVGFESLEECYMAKILVAEGTRDVPIGAIICITVGKPEDIEAFKNYTLDSSAAPTPQAAPAPTPAATASPPTPSAQAPGSSYPPHMQVLLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEGYLAKILVPEGTRDVPLGTPLCIIVEKEADISAFADYRPTEVTDLKPQVPPPT

• Full Name: dihydrolipoamide S-acetyltransferase
• Calculated Molecular Weight: 647 aa, 70 kDa
• Observed Molecular Weight: 69-70 kDa
• GenBank Accession Number: BC039084
• Gene Symbol: DLAT
• Gene ID (NCBI): 1737
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P10515
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.