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  • Western Blot (WB) analysis of various lysates using ZMAT3 Monoclonal antibody (68346-1-Ig)

    WB analysis using 68346-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68346-1-Ig (ZMAT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ZMAT3 Monoclonal antibody (68346-1-Ig)

    WB analysis using 68346-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68346-1-Ig (ZMAT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ZMAT3 Monoclonal antibody (68346-1-Ig)

    WB analysis using 68346-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68346-1-Ig (ZMAT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

ZMAT3 Monoclonal antibody

ZMAT3 Monoclonal Antibody for WB, ELISA
Cat No. 68346-1-Ig
Clone No.2D2E7

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Synonyms

FLJ12296, p53 activated gene 608 protein, PAG608, WIG 1, WIG1, Zinc finger protein WIG 1, zinc finger, matrin type 3, ZMAT3

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inCOLO 320 cells, HEK-293 cells, U2OS cells, HepG2 cells, K-562 cells, hTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68346-1-Ig targets ZMAT3 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag20552

Product name: Recombinant human ZMAT3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-289 aa of BC002896

Sequence: MILLQHAVLPPPKQPSPSPPMSVATRSTGTLQLPPQKPFGQEASLPLAGEEELSKGGEQDCALEELCKPLYCKLCNVTLNSAQQAQAHYQGKNHGKKLRNYYAANSCPPPARMSNVVEPAATPVVPVPPQMGSFKPGGRVILATENDYCKLCDASFSSPAVAQAHYQGKNHAKRLRLAEAQSNSFSESSELGQRRARKEGNEFKMMPNRRNMYTVQNNSAGPYFNPRSRQRIPRDLAMCVTPSGQFYCSMCNVGAGEEMEFRQHLESKQHKSKVSEQRYRNEMENLGYV

Predict reactive species
Full Name zinc finger, matrin type 3
Calculated Molecular Weight 32 kDa
Observed Molecular Weight~32 kDa
GenBank Accession NumberBC002896
Gene Symbol ZMAT3
Gene ID (NCBI) 64393
RRIDAB_3085069
Conjugate Unconjugated
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9HA38
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZMAT3 is a p53-induced zinc finger protein, and a role in the TP53-dependent growth regulatory pathway, contribute to TP53-mediated apoptosis by regulation of TP53 expression and translocation to the nucleus and nucleolus. Acts as a bona fide target gene of p53/TP53.

Protocols

Product Specific Protocols
WB protocol for ZMAT3 antibody 68346-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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