ZMAT3 Monoclonal antibody, PBS Only (Detector)

• 

  WB analysis using 68346-1-Ig (same clone as 68346-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68346-1-Ig (ZMAT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68346-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68346-1-Ig (same clone as 68346-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68346-1-Ig (ZMAT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68346-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68346-1-Ig (same clone as 68346-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68346-1-Ig (ZMAT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68346-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50120-1

  Cytometric bead array standard curve of MP50120-1, ZMAT3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68346-2-PBS. Detection antibody: 68346-1-PBS. Standard:Ag20552. Range: 3.125-100 ng/mL.

ZMAT3 Monoclonal Antibody for WB, Indirect ELISA, Cytometric bead array

Cat No. 68346-1-PBS

Clone No.2D2E7

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA, Cytometric bead array

Synonyms

FLJ12296, p53 activated gene 608 protein, PAG608, WIG 1, WIG1, Zinc finger protein WIG 1, zinc finger, matrin type 3, ZMAT3

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Product Information

68346-1-PBS targets ZMAT3 as part of a matched antibody pair:

MP50120-1: 68346-2-PBS capture and 68346-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20552

  Product name: Recombinant human ZMAT3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-289 aa of BC002896

  Sequence: MILLQHAVLPPPKQPSPSPPMSVATRSTGTLQLPPQKPFGQEASLPLAGEEELSKGGEQDCALEELCKPLYCKLCNVTLNSAQQAQAHYQGKNHGKKLRNYYAANSCPPPARMSNVVEPAATPVVPVPPQMGSFKPGGRVILATENDYCKLCDASFSSPAVAQAHYQGKNHAKRLRLAEAQSNSFSESSELGQRRARKEGNEFKMMPNRRNMYTVQNNSAGPYFNPRSRQRIPRDLAMCVTPSGQFYCSMCNVGAGEEMEFRQHLESKQHKSKVSEQRYRNEMENLGYV

• Full Name: zinc finger, matrin type 3
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: ~32 kDa
• GenBank Accession Number: BC002896
• Gene Symbol: ZMAT3
• Gene ID (NCBI): 64393
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9HA38
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ZMAT3 is a p53-induced zinc finger protein, and a role in the TP53-dependent growth regulatory pathway, contribute to TP53-mediated apoptosis by regulation of TP53 expression and translocation to the nucleus and nucleolus. Acts as a bona fide target gene of p53/TP53.