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Tuberin/TSC2 Monoclonal antibody (68380-1-Ig)

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KD/KO Validated

Tuberin/TSC2 Monoclonal antibody

Tuberin/TSC2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 68380-1-Ig

Clone No. 1A10B2

Publications (1)

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Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA
For FC, select conjugated version.

Synonyms

TSC2, Tuberin, 1A10B2, TSC4, tuberous sclerosis 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

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Filter: AllWBIHCIF/ICC

WB analysis using 68380-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 68380-1-Ig
WB result of Tuberin/TSC2 antibody (68380-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Tuberin/TSC2 transfected HeLa cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of MCF-7 using 68380-1-Ig
MCF-7 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of PC-3 using 68380-1-Ig
PC-3 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of NIH/3T3 using 68380-1-Ig
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HSC-T6 using 68380-1-Ig
HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat brain using 68380-1-Ig
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68380-1-Ig (Tuberin/TSC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat brain using 68380-1-Ig
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68380-1-Ig (Tuberin/TSC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung cancer using 68380-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68380-1-Ig (Tuberin/TSC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung cancer using 68380-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68380-1-Ig (Tuberin/TSC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HEK-293 using 68380-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TSC2 antibody (68380-1-Ig, Clone: 1A10B2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for Tuberin/TSC2 in ?

Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	A549 cells, MCF-7 cells, PC-3 cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells, U2OS cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IHC detected in	rat brain tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68380-1-Ig targets Tuberin/TSC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	tuberous sclerosis 2
Calculated Molecular Weight	1807 aa, 201 kDa
Observed Molecular Weight	200 kDa
GenBank Accession Number	BC150300
Gene Symbol	TSC2
Gene ID (NCBI)	7249
RRID	AB_3085098
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P49815
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TSC2, also named Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling.

Protocols

Product Specific Protocols
IF protocol for Tuberin/TSC2 antibody 68380-1-Ig	Download protocol
IHC protocol for Tuberin/TSC2 antibody 68380-1-Ig	Download protocol
WB protocol for Tuberin/TSC2 antibody 68380-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The Tuberin/TSC2 Monoclonal antibody (1A10B2), catalog number 68380-1-Ig, has a total of 1 citation. It appears in Nature Cancer (Impact Factor: 28), published in January 2026. The cited study, titled "CDK10 suppresses nucleic acid sensors-mediated antitumor immunity," falls within the research fields of cancer biology and innate immune signaling, specifically exploring the role of CDK10 in regulating nucleic acid sensor pathways and antitumor immune responses.