Tuberin/TSC2 Monoclonal antibody, PBS Only

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  WB analysis using 68380-1-Ig (same clone as 68380-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68380-1-Ig (same clone as 68380-1-PBS)

  WB result of Tuberin/TSC2 antibody (68380-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Tuberin/TSC2 transfected HeLa cells. This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 68380-1-Ig (same clone as 68380-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 68380-1-Ig (same clone as 68380-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 68380-1-Ig (same clone as 68380-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 68380-1-Ig (same clone as 68380-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 68380-1-Ig (same clone as 68380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68380-1-Ig (Tuberin/TSC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 68380-1-Ig (same clone as 68380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68380-1-Ig (Tuberin/TSC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 68380-1-Ig (same clone as 68380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68380-1-Ig (Tuberin/TSC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 68380-1-Ig (same clone as 68380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68380-1-Ig (Tuberin/TSC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 68380-1-Ig (same clone as 68380-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TSC2 antibody (68380-1-Ig, Clone: 1A10B2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

Tuberin/TSC2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 68380-1-PBS

Clone No.1A10B2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

TSC2, Tuberin, 1A10B2, TSC4, tuberous sclerosis 2

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Product Information

68380-1-PBS targets Tuberin/TSC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: tuberous sclerosis 2
• Calculated Molecular Weight: 1807 aa, 201 kDa
• Observed Molecular Weight: 200 kDa
• GenBank Accession Number: BC150300
• Gene Symbol: TSC2
• Gene ID (NCBI): 7249
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P49815
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TSC2, also named Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling.