NVL Monoclonal antibody

NVL Monoclonal Antibody for WB, FC (Intra), ELISA

Cat No. 68399-1-Ig

Clone No. 1D8G4

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Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

1D8G4, Nuclear valosin-containing protein-like, nuclear VCP like, Nuclear VCP like protein, Nuclear VCP-like protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

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Filter: AllWBFC (Intra)

WB analysis using 68399-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 68399-1-Ig (NVL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of HeLa using 68399-1-Ig
1x10^6 HeLa cells were intracellularly stained with 0.2 ug NVL Monoclonal antibody (68399-1-Ig, Clone:1D8G4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.2 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
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Tested Applications

Positive WB detected in	HepG2 cells, HeLa cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68399-1-Ig targets NVL in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag9321 Product name: Recombinant human NVL protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 309-659 aa of BC012105 Sequence: LPSKGVQEERLGTEPTSETQDELQRLLGLLRDQDPLSEEQMQGLCIELNDFIVALSSVQPSAKREGFVTVPNVTWADIGALEDIREELTMAILAPVRNPDQFKALGLVTPAGVLLAGPPGCGKTLLAKAVANESGLNFISVKGPELLNMYVGESERAVRQVFQRAKNSAPCVIFFDEVDALCPRRSDRETGASVRVVNQLLTEMDGLEARQQVFIMAATNRPDIIDPAILRPGRLDKTLFVGLPPPADRLAILKTITKNGTKPPLDADVNLEAIAGDLRCDCYTGADLSALVREASICALRQEMARQKSGNEKGELKVSHKHFEEAFKKVRSSISKKDQIMYERLQESLSR Predict reactive species
Full Name	nuclear VCP-like
Calculated Molecular Weight	856aa,95 kDa; 659aa,73 kDa
Observed Molecular Weight	95 kDa
GenBank Accession Number	BC012105
Gene Symbol	NVL
Gene ID (NCBI)	4931
RRID	AB_3085116
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	O15381
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NVL(nuclear VCP-like) , also named as NVL2, belongs to the AAA ATPase family and may facilitate the dissociation and recycling of nucleolin, thereby promoting efficient ribosome biogenesis. It acts as an ATP-dependent zinc metallopeptidase. NVL plays an essential role in ribosome biogenesis by specifically associating with ribosomal protein L5 and modulating the function of DOB1, thereby participating in the assembly of the 60S ribosomal subunit. Additionally, NVL is required for telomerase assembly and the regulation of telomerase activity, and is involved in pre-rRNA processing. This protein has 5 isoforms produced by alternative splicing.