NVL Monoclonal antibody, PBS Only

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  WB analysis using 68399-1-Ig (same clone as 68399-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68399-1-Ig (NVL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68399-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 68399-1-Ig (same clone as 68399-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.2 ug NVL Monoclonal antibody (68399-1-Ig, Clone:1D8G4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.2 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68399-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 68399-1-PBS

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug NVL Monoclonal antibody, PBS Only (68399-1-PBS, Clone:1D8G4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

NVL Monoclonal Antibody for WB, FC (Intra), Indirect ELISA

Cat No. 68399-1-PBS

Clone No.1D8G4

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

1D8G4, Nuclear valosin-containing protein-like, nuclear VCP like, Nuclear VCP like protein, Nuclear VCP-like protein

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Tested Applications

• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68399-1-PBS targets NVL in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9321

  Product name: Recombinant human NVL protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 309-659 aa of BC012105

  Sequence: LPSKGVQEERLGTEPTSETQDELQRLLGLLRDQDPLSEEQMQGLCIELNDFIVALSSVQPSAKREGFVTVPNVTWADIGALEDIREELTMAILAPVRNPDQFKALGLVTPAGVLLAGPPGCGKTLLAKAVANESGLNFISVKGPELLNMYVGESERAVRQVFQRAKNSAPCVIFFDEVDALCPRRSDRETGASVRVVNQLLTEMDGLEARQQVFIMAATNRPDIIDPAILRPGRLDKTLFVGLPPPADRLAILKTITKNGTKPPLDADVNLEAIAGDLRCDCYTGADLSALVREASICALRQEMARQKSGNEKGELKVSHKHFEEAFKKVRSSISKKDQIMYERLQESLSR

• Full Name: nuclear VCP-like
• Calculated Molecular Weight: 856aa,95 kDa; 659aa,73 kDa
• Observed Molecular Weight: 95 kDa
• GenBank Accession Number: BC012105
• Gene Symbol: NVL
• Gene ID (NCBI): 4931
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O15381
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

NVL(nuclear VCP-like) belongs to the AAA ATPase family and may facilitate the dissociation and recycling of nucleolin, thereby promoting efficient ribosome biogenesis. It acts as an ATP-dependent zinc metallopeptidase. This protein has 5 isoforms produced by alternative splicing.