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  • Western Blot (WB) analysis of various lysates using TFAM Monoclonal antibody (68557-5-Ig)

    WB analysis using 68557-5-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68557-5-Ig (TFAM antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TFAM Monoclonal antibody (68557-5-Ig)

    WB analysis using 68557-5-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68557-5-Ig (TFAM antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TFAM Monoclonal antibody (68557-5-Ig)

    IHC staining of human colon cancer using 68557-5-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68557-5-Ig (TFAM antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TFAM Monoclonal antibody (68557-5-Ig)

    IHC staining of human colon cancer using 68557-5-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68557-5-Ig (TFAM antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TFAM Monoclonal antibody (68557-5-Ig)

    IF Staining of HepG2 using 68557-5-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TFAM antibody (68557-5-Ig, Clone: 1C8H3 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

TFAM Monoclonal antibody

TFAM Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68557-5-Ig
Clone No.1C8H3

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

MtTF1, mtTFA, TCF6, TCF-6, Transcription factor 6-like 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inJurkat cells, Caco-2 cells, U2OS cells, LNCaP cells, MOLT-4 cells, MJ cells, K-562 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68557-5-Ig targets TFAM in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TFAM fusion protein Ag18253 Predict reactive species
Full Name transcription factor A, mitochondrial
Calculated Molecular Weight 246 aa, 29 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC126366
Gene Symbol TFAM
Gene ID (NCBI) 7019
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ00059
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3, and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.

Protocols

Product Specific Protocols
WB protocol for TFAM antibody 68557-5-IgDownload protocol
IHC protocol for TFAM antibody 68557-5-IgDownload protocol
IF protocol for TFAM antibody 68557-5-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols