Tested Applications
| Positive WB detected in | A549 cells, HAP1 cells, Jurkat cells, rat colon tissue, HeLa cells, K-562 cells, pig colon tissue, rabbit colon tissue, mouse colon tissue, mouse small intestine tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68561-1-Ig targets Integrin alpha-5/CD49e in WB, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag33642 Product name: Recombinant human Integrin alpha-5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 749-1049 aa of BC008786 Sequence: FTVPHLRDTKKTIQFDFQILSKNLNNSQSDVVSFRLSVEAQAQVTLNGVSKPEAVLFPVSDWHPRDQPQKEEDLGPAVHHVYELINQGPSSISQGVLELSCPQALEGQQLLYVTRVTGLNCTTNHPINPKGLELDPEGSLHHQQKREAPSRSSASSGPQILKCPEAECFRLRCELGPLHQQESQSLQLHFRVWAKTFLQREHQPFSLQCEAVYKALKMPYRILPRQLPQKERQVATAVQWTKAEGSYGVPLWIIILAILFGLLLLGLLIYILYKLGFFKRSLPYGTAMEKAQLKPPATSDA Predict reactive species |
| Full Name | integrin, alpha 5 (fibronectin receptor, alpha polypeptide) |
| Calculated Molecular Weight | 115 kDa |
| Observed Molecular Weight | 140 kDa |
| GenBank Accession Number | BC008786 |
| Gene Symbol | Integrin alpha 5 |
| Gene ID (NCBI) | 3678 |
| RRID | AB_3085263 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P08648 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha 5 (ITGA5), belongs to the integrin alpha chain family, primarily binds to Integrin beta 1 (ITGB1) to form an α5β1 heterodimer (PMID: 33711961). Integrin α5β1 is a specific receptor of fibronectin through its arginine-glycine-aspartic acid (RGD) binding site (PMID: 19608542). Integrin α5β1 plays roles in cell adhesion, migration and matrix formation, and has emerged as an essential mediator in many human carcinomas (PMID: 33408483).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Integrin alpha-5/CD49e antibody 68561-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Integrin alpha-5/CD49e Monoclonal antibody (3B9E5), catalog number 68561-1-Ig, has 3 total citations. They appear in Nature Communications, Acta Pharmacologica Sinica, and Genomics. The associated research fields include synthetic biology and 3D cell culture engineering, cardiovascular pharmacology (angiogenesis and myocardial infarction), and human genetics (childhood absence epilepsy).
| Species | Application | Title |
|---|---|---|
Nat Commun Self-assembly of hybrid 3D cultures by integrating living and synthetic cells. | ||
Acta Pharmacol Sin Resibufogenin promotes angiogenesis via the VAV3-mediated ITGA5-VEGF signaling axis in macrophages to alleviate myocardial infarction. | ||
Genomics The noncausal association between a loss-of-function CLCN2 variant and childhood absence epilepsy |
