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Pseudouridine Monoclonal antibody (68578-1-Ig)

Pseudouridine Monoclonal antibody

Pseudouridine Monoclonal Antibody for Dot Blot, ELISA

Cat No. 68578-1-Ig

Clone No.1G7C7

Publications(3)

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

chemical compound and More (1)

Applications

Dot Blot, ELISA

Synonyms

Ψ, 5-ribosyluracil

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

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Filter: AllELISADot Blot

ELISA experiment of Pseudouridine using 68578-1-Ig
Indirect ELISA and competitive ELISA results show that this antibody is specific to Pseudouridine. Indirect ELISA was performed by coating BSA conjugated Pseudouridine at 10ng/well followed by blocking with 1% BSA. Serial diluted primary antibody was added to the plates and incubated at 37℃. HRP-goat anti-mouse was used for detection. Competitive ELISA was performed similarly except that different concentration of Pseudouridine or its structure analogue compounds are mixed in primary antibody (fixed at 10 ng/mL).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
Indirect ELISA and competitive ELISA results show that this antibody is specific to Pseudouridine. Indirect ELISA was performed by coating BSA conjugated Pseudouridine at 10ng/well followed by blocking with 1% BSA. Serial diluted primary antibody was added to the plates and incubated at 37℃. HRP-goat anti-mouse was used for detection. Competitive ELISA was performed similarly except that different concentration of Pseudouridine or its structure analogue compounds are mixed in primary antibody (fixed at 10 ng/mL).
Dot Blot experiment of RNA using 68578-1-Ig
Total RNA was isolated from HeLa cell line and was dotted to NC membrane at different amount as indicated above the dots. The membrane was blocked with BSA and blotted with Pseudouridine antibody 68578-1-Ig at 1:2000 followed by incubation of HRP-goat anti-mouse secondary antibody. Signal was developed by ECL substrate. A parallel dot blot was performed using mouse IgG1 isotype control antibody 66360-1-Ig at the same dose.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
Total RNA was isolated from HeLa cell line and was dotted to NC membrane at different amount as indicated above the dots. The membrane was blocked with BSA and blotted with Pseudouridine antibody 68578-1-Ig at 1:2000 followed by incubation of HRP-goat anti-mouse secondary antibody. Signal was developed by ECL substrate. A parallel dot blot was performed using mouse IgG1 isotype control antibody 66360-1-Ig at the same dose.

Overview

Tested Applications
Product Information
Publications (3)

Print datasheet

MSDS SDS

Tested Applications

Positive ELISA detected in	Pseudouridine
Positive Dot Blot detected in	RNA

Recommended dilution

Application	Dilution
Enzyme-linked Immunosorbent Assay (ELISA)	ELISA : 1:2000-1:20000
DOT BLOT	DOT BLOT : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68578-1-Ig targets Pseudouridine in Dot Blot, ELISA applications and shows reactivity with chemical compound samples.

Tested Reactivity	chemical compound
Cited Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	PTG Predict reactive species
Full Name	Pseudouridine
Gene Symbol
Gene ID (NCBI)
RRID	AB_3085278
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pseudouridine is an isomer of the nucleoside uridine in which the uracil is attached via a carbon-carbon instead of a nitrogen-carbon glycosidic bond. Pseudouridine is the most abundant RNA modification in cellular RNA (PMID: 29104216). After transcription and following synthesis, RNA can be modified with over 100 chemically distinct modifications. These can potentially regulate RNA expression post-transcriptionally, in addition to the four standard nucleotides and play a variety of roles in the cell including translation, localization and stabilization of RNA. Pseudouridine in rRNA and tRNA has been shown to fine-tune and stabilize the regional structure and help maintain their functions in mRNA decoding, ribosome assembly, processing and translation (PMID: 10902565, PMID: 23391857, PMID: 28045575). Pseudouridine in snRNA has been shown to enhance spliceosomal RNA-pre-mRNA interaction to facilitate splicing regulation (PMID 27268497).

Protocol for Dot Blot:
https://www.ptglab.com/protocol/68578-1-IgDotBlot.pdf

Publications

What published studies show

All (3)

Species	Application	Title
human		Cell Signal PUS1 facilitates cell migration in clear cell renal cell carcinoma through the promotion of mRNA pseudouridylation and the stabilization of the SMOX gene Authors - Yao Chen View Article
		3 Biotech Arecoline promotes oral submucous fibrosis by enhancing epithelial cell cuproptosis through PUS1-mediated pseudouridylation. Authors - Shigeng Lin View Article
		Gastroenterol Rep (Oxf) TRUB1 is a novel biomarker for promoting malignancy in colorectal cancer via NFκB signaling Authors - Yingzhao Wang View Article