Na Pseudouridine Monoclonal antibody, PBS Only

• 

  ELISA experiment of Pseudouridine using 68578-1-Ig (same clone as 68578-1-PBS)

  Indirect ELISA and competitive ELISA results show that this antibody is specific to Pseudouridine. Indirect ELISA was performed by coating BSA conjugated Pseudouridine at 10ng/well followed by blocking with 1% BSA. Serial diluted primary antibody was added to the plates and incubated at 37℃. HRP-goat anti-mouse was used for detection. Competitive ELISA was performed similarly except that different concentration of Pseudouridine or its structure analogue compounds are mixed in primary antibody (fixed at 10 ng/mL). This data was developed using the same antibody clone with 68578-1-PBS in a different storage buffer formulation.

• 

  Dot Blot experiment of RNA using 68578-1-Ig (same clone as 68578-1-PBS)

  Total RNA was isolated from HeLa cell line and was dotted to NC membrane at different amount as indicated above the dots. The membrane was blocked with BSA and blotted with Pseudouridine antibody 68578-1-Ig at 1:2000 followed by incubation of HRP-goat anti-mouse secondary antibody. Signal was developed by ECL substrate. A parallel dot blot was performed using mouse IgG1 isotype control antibody 66360-1-Ig at the same dose. This data was developed using the same antibody clone with 68578-1-PBS in a different storage buffer formulation.

Pseudouridine Monoclonal Antibody for Dot Blot, ELISA, Indirect ELISA

Cat No. 68578-1-PBS

Clone No.1G7C7

Host / Isotype

Mouse / IgG1

Reactivity

chemical compound

Applications

Dot Blot, ELISA, Indirect ELISA

Synonyms

Ψ, 5-ribosyluracil

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Product Information

68578-1-PBS targets Pseudouridine in Dot Blot, ELISA, Indirect ELISA applications and shows reactivity with chemical compound samples.

• Tested Reactivity: chemical compound
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  PTG

• Full Name: Pseudouridine
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Pseudouridine is an isomer of the nucleoside uridine in which the uracil is attached via a carbon-carbon instead of a nitrogen-carbon glycosidic bond. Pseudouridine is the most abundant RNA modification in cellular RNA (PMID: 29104216). After transcription and following synthesis, RNA can be modified with over 100 chemically distinct modifications. These can potentially regulate RNA expression post-transcriptionally, in addition to the four standard nucleotides and play a variety of roles in the cell including translation, localization and stabilization of RNA. Pseudouridine in rRNA and tRNA has been shown to fine-tune and stabilize the regional structure and help maintain their functions in mRNA decoding, ribosome assembly, processing and translation (PMID: 10902565, PMID: 23391857, PMID: 28045575). Pseudouridine in snRNA has been shown to enhance spliceosomal RNA-pre-mRNA interaction to facilitate splicing regulation (PMID 27268497).

Protocol for Dot Blot:
https://www.ptglab.com/protocol/68578-1-IgDotBlot.pdf