Tested Applications
| Positive WB detected in | pig liver tissue, mouse liver tissue, mouse brain tissue, pig spleen tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68604-1-Ig targets RSAD2 in WB, IF, ELISA applications and shows reactivity with human, mouse, pig samples.
| Tested Reactivity | human, mouse, pig |
| Cited Reactivity | mouse, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27733 Product name: Recombinant human RSAD2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 181-361 aa of BC017969 Sequence: DSFDEEVNVLIGRGQGKKNHVENLQKLRRWCRDYRVAFKINSVINRFNVEEDMTEQIKALNPVRWKVFQCLLIEGENCGEDALREAERFVIGDEEFERFLERHKEVSCLVPESNQKMKDSYLILDEYMRFLNCRKGRKDPSKSILDVGVEEAIKFSGFDEKMFLKRGGKYIWSKADLKLDW Predict reactive species |
| Full Name | radical S-adenosyl methionine domain containing 2 |
| Calculated Molecular Weight | 361 aa, 42 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | BC017969 |
| Gene Symbol | RSAD2 |
| Gene ID (NCBI) | 91543 |
| RRID | AB_3085300 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q8WXG1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RSAD2 (radical S-adenosyl methionine domain-containing protein 2), also known as CIG5 (cytomegalovirus-induced gene 5 protein), vig1, viperin or CIG33, displays antiviral effect against HIV-1 virus, hepatitis C virus, human cytomegalovirus, and aphaviruses. Expression of the protein can be induced by interferon.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RSAD2 antibody 68604-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RSAD2 Monoclonal antibody (1C5D4), catalog number 68604-1-Ig, has 5 total citations. These appear in International Journal of Biological Macromolecules, Journal of Immunology, Veterinary Microbiology (two papers), and Virulence. The research fields span antiviral immunity and viral replication (coronavirus, Senecavirus A, PDCoV), type I interferon signaling, cGAS-STING/NF-κB pathways, mitochondrial protection in microglia, and lipid droplet dynamics in viral suppression.
| Species | Application | Title |
|---|---|---|
Int J Biol Macromol Viperin inhibits coronavirus replication by targeting non-structural protein 8 to disrupt replication-transcription complex assembly and RNA-dependent RNA polymerase activity. | ||
J Immunol LRRK2 kinase activity restricts NRF2-dependent mitochondrial protection in microglia. | ||
Vet Microbiol RSAD2 inhibits Senecavirus A replication by promoting type I interferons induction downstream of cGAS-STING and NF-κB signaling pathway. | ||
Vet Microbiol Lipid droplet dynamics control type I interferon responses and viperin expression to suppress viral replication. | ||
Virulence Integrated multi-omics analysis identifies PPAR signaling network associations with metabolic-immune dysregulation and intestinal injury in PDCoV-infected piglets. |
