TCF4/ITF2 Monoclonal antibody

TCF4/ITF2 Monoclonal Antibody for WB, ELISA

Cat No. 68607-1-Ig

Clone No. 1C9A1

Publications (1)

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Host / Isotype

Mouse / IgG1

Reactivity

human, rat and More (1)

Applications

WB, ELISA

Synonyms

TCF4, 1C9A1, bHLHb19, Class B basic helix-loop-helix protein 19, E2 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

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WB analysis using 68607-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 68607-1-Ig (TCF4/ITF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in

U2OS cells, HCT 116 cells, Caco-2 cells, A549 cells, PC-12 cells, SH-SY5Y cells, K-562 cells, Daudi cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68607-1-Ig targets TCF4/ITF2 in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity	human, rat
Cited Reactivity	chicken
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag17897 Product name: Recombinant human TCF4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 407-560 aa of BC125084 Sequence: PSTAMPGGHGDMHGIIGPSHNGAMGGLGSGYGTGLLSANRHSLMVGTHREDGVALRGSHSLLPNQVPVPQLPVQSATSPDLNPPQDPYRGMPPGLQGQSVSSGSSEIKSDDEGDENLQDTKSSEDKKLDDDKKDIKSITRSRSSNNDDEDLTPE Predict reactive species
Full Name	transcription factor 4
Calculated Molecular Weight	671 aa, 72 kDa
Observed Molecular Weight	72 kDa; 68 kDa
GenBank Accession Number	BC125084
Gene Symbol	TCF4
Gene ID (NCBI)	6925
RRID	AB_3085302
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P15884
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TCF4/ITF2 antibody 68607-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The TCF4/ITF2 Monoclonal antibody (Cat# 68607-1-Ig) has 1 citation to date. It was cited in iScience (IF 4.5) in a 2026 study by Fang Wang et al., which investigated how late ad libitum feeding reverses early feed restriction-induced immune deficits in broilers. The antibody was applied in Western Blot (WB) using chicken samples, placing the research in the fields of poultry nutrition and immunology.