Tested Applications
| Positive WB detected in | pig kidney tissue, rabbit kidney tissue |
| Positive IHC detected in | rat adrenal gland tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68719-1-Ig targets ALPL in WB, IHC, IF, ELISA applications and shows reactivity with human, pig, rabbit samples.
| Tested Reactivity | human, pig, rabbit |
| Cited Reactivity | human, mouse, rat, pig, rabbit |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag33678 Product name: Recombinant human ALPL protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 23-330 aa of BC021289 Sequence: EKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFEPGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLL Predict reactive species |
| Full Name | alkaline phosphatase, liver/bone/kidney |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 80 kDa |
| GenBank Accession Number | BC021289 |
| Gene Symbol | ALPL |
| Gene ID (NCBI) | 249 |
| RRID | AB_3670412 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P05186 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme that is expressed in multiple tissues including liver, bone, and kidney, rather than being restricted to a single tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. ALPL(Alkaline phosphatase, tissue-nonspecific isozyme) is also named as AP-TNAP, TNSALP and belongs to the alkaline phosphatase family. It can exsit as a homodimer with the molecular weight of 140 kDa(PMID:18724009). ALPL was synthesized as a 66-kDa endo-b-N-acetylglucos-aminidase H (Endo H)-sensitive form, and processed to an 80-kDa mature form, which is anchored to the plasma membrane via glycosylphosphatidylinositol (GPI)(PMID:10839996).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ALPL antibody 68719-1-Ig | Download protocol |
| WB protocol for ALPL antibody 68719-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ALPL Monoclonal antibody (2E3C1), catalog number 68719-1-Ig, has 6 total citations. These appear in journals including Advanced Healthcare Materials, International Journal of Biological Macromolecules, International Endodontic Journal, Noncoding RNA Research, Biochemical and Biophysical Research Communications, and Journal of Cellular and Molecular Medicine. The research fields span bone regeneration, dentine regeneration, hair follicle growth, periodontal bone repair, and age-related osteoporosis.
| Species | Application | Title |
|---|---|---|
Adv Healthc Mater Masticatory-Driven Piezoelectric Hydrogels with Electrical Stimulation-Triggered NPY Condensate for Mandibular Bone Regeneration. | ||
Int J Biol Macromol Near-infrared responsive nanozyme-carboxymethyl chitosan/chondroitin sulfate composite hydrogel: Multicomponent synergy optimizes bone defect microenvironment to enhance repair. | ||
Int Endod J Injectable Thermo-Responsive Dentine Matrix Hydrogel for Enhancing Dentine Regeneration. | ||
Noncoding RNA Res Exosomal miRNA-218-5p derived from low-passage dermal papilla cells modulates hair follicle growth and development. | ||
Biochem Biophys Res Commun GATA-1-driven TOMM20-mediated mitophagy regulates PDLSCs osteogenesis and enhances periodontal bone regeneration. | ||
J Cell Mol Med Prmt6 Deficiency Drives Osteoblast Senescence Promoting Age-Related Osteoporosis via Epigenetic Remodelling of the H3R2me2a/Sting/Ifitm3 Pathway. |
