Tested Applications
| Positive WB detected in | HeLa cells, HAP1 cells, K-562 cells, Neuro-2a cells, C6 cells |
| Positive IHC detected in | human gliomas tissue, rat cerebellum tissue, mouse brain tissue, human brain tissue, human gliomas tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat brain tissue, HAP1 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:300-1:1200 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
80001-1-RR targets TDP-43 (N-terminal) in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Recombinant protein Predict reactive species |
| Full Name | TAR DNA binding protein |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 43 kDa |
| GenBank Accession Number | BC001487 |
| Gene Symbol | TDP-43 |
| Gene ID (NCBI) | 23435 |
| RRID | AB_2882933 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13148 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene.
In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS).
Proteintech's 80001-1-RR is a rabbit recombinant TDP-43 antibody recognizing N-terminal TDP-43. It recognizes the intact 43 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659, 19823856, 21666678, 22193176)
Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (PMID: 29581274).
80001-1-RR can be used in WB, IHC, IHF. For the ICC (IF with cells) experiment, the results will be better with ethanol fixed cell and dilution 1:50 or 1:100. Another antibody with CatNo. 80002-1-RR is recommended for IF or ICC experiment.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for TDP-43 (N-terminal) antibody 80001-1-RR | Download protocol |
| IF protocol for TDP-43 (N-terminal) antibody 80001-1-RR | Download protocol |
| IHC protocol for TDP-43 (N-terminal) antibody 80001-1-RR | Download protocol |
| WB protocol for TDP-43 (N-terminal) antibody 80001-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TDP-43 antibody (Cat# 80001-1-RR) has 5 citations. These appear in Nature Neuroscience, Brain Pathology, Journal of Neuropathology & Experimental Neurology, Neuroreport, and F1000Research. Research fields span ALS and neurodegeneration, TDP-43 pathology and aggregation, neurotoxicity, and antibody validation for WB, IP, and IF applications, covering both human and mouse specimens.
| Species | Application | Title |
|---|---|---|
Nat Neurosci Integrated single-cell and spatial transcriptomic profiling in ALS uncovers peripheral-to-central immune infiltration and reprogramming. | ||
Brain Pathol Nicorandil treatment improves survival and spatial learning in aged granulin knockout mice | ||
J Neuropathol Exp Neurol Locus coeruleus TDP-43 pathology in a community-based cohort: Clinical and pathological correlates. | ||
Neuroreport Glutaredoxin-1 attenuates transactive response DNA-binding protein 43-induced neurotoxicity by suppressing oxidative stress and transactive response DNA-binding protein 43 aggregation. | ||
F1000Res The identification of high-performing antibodies for TDP-43 for use in Western Blot, immunoprecipitation and immunofluorescence |
Reviews
What customers say
The antibody performed well in IHC on ethanol and NBF-fixed human brain and spinal cord sections, including control, FTLD-TDP, and ALS cases. It reliably detected normal nuclear TDP-43 as well as cytoplasmic inclusions, particularly in ALS spinal cord. Both citrate buffer and formic acid worked effectively as antigen retrieval solutions. Optimal dilutions of 1:5,000 to 1:10,000 provided high specificity with low background. Overall, the reviewer rated it 4 out of 5, describing it as a good, reliable antibody for TDP-43 detection.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Silvia (Verified Customer) (02-20-2020) | Good antibody. I have used it to stain both Ethanol and NBF fixed human Control and FTLD-TDP brain section and spinal cord from ALS cases by IHC. I have tested either citrate buffer or FA as antigen retrieval solutions, and in both conditions nicely detected the normal nuclear TDP-43 as well as cytoplasmic inclusions specially in spinal cord form ALS cases . The dilutions that worked better showing high specificity and low background ranged from 5,000-10,000.
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