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  • Western Blot (WB) analysis of HEK-293 cells using Phospho-P62/SQSTM1 (Ser349) Recombinant antibody (80294-2-RR)

    WB analysis of HEK-293 using 80294-2-RR

    Non-treated HEK-293 cells and sodium arsenite treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80294-2-RR (Phospho-P62/SQSTM1 (Ser349) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using Phospho-P62/SQSTM1 (Ser349) Recombinant antibody (80294-2-RR)

    WB analysis of NIH/3T3 using 80294-2-RR

    Non-treated NIH/3T3 cells and MG132 treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80294-2-RR (Phospho-P62/SQSTM1 (Ser349) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

Phospho-P62/SQSTM1 (Ser349) Recombinant antibody

Phospho-P62/SQSTM1 (Ser349) Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 80294-2-RR
Clone No.250277E9

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

Synonyms

P62, P62/SQSTM1, SQSTM1, EBI3-associated protein of 60 kDa, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected insodium arsenite treated HEK-293 cells, MG132 treated NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80294-2-RR targets Phospho-P62/SQSTM1 (Ser349) in applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide Predict reactive species
Full Name sequestosome 1
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberBC017222
Gene Symbol P62/SQSTM1
Gene ID (NCBI) 8878
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13501
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. It functions as a bridge between polyubiquitinated cargo and autophagosomes (PMID:16286508). SQSTM1 is at the cross-roads of several signaling pathways including Keap1-Nrf2 pathway , NFκB pathway , NFE2L2/NRF2 pathway , mTOR pathway and Wnt pathway. Phosphorylation and/or de-phosphorylation of p62-Ser349 may participate in the regulation of both selective autophagy and oxidative stress response (PMID: 33397898).

Protocols

Product Specific Protocols
WB protocol for Phospho-P62/SQSTM1 (Ser349) antibody 80294-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols