Phospho-P62/SQSTM1 (Ser349) Recombinant antibody, PBS Only

• 

  WB analysis of HEK-293 using 80294-2-RR (same clone as 80294-2-PBS)

  Non-treated HEK-293 cells and sodium arsenite treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80294-2-RR (Phospho-P62/SQSTM1 (Ser349) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80294-2-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 80294-2-RR (same clone as 80294-2-PBS)

  Non-treated NIH/3T3 cells and MG132 treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80294-2-RR (Phospho-P62/SQSTM1 (Ser349) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80294-2-PBS in a different storage buffer formulation.

Phospho-P62/SQSTM1 (Ser349) Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Cat No. 80294-2-PBS

Clone No.250277E9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

Synonyms

P62, P62/SQSTM1, SQSTM1, EBI3-associated protein of 60 kDa, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa

---

Product Information

80294-2-PBS targets Phospho-P62/SQSTM1 (Ser349) in applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: sequestosome 1
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC017222
• Gene Symbol: P62/SQSTM1
• Gene ID (NCBI): 8878
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13501
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. It functions as a bridge between polyubiquitinated cargo and autophagosomes (PMID:16286508). SQSTM1 is at the cross-roads of several signaling pathways including Keap1-Nrf2 pathway , NFκB pathway , NFE2L2/NRF2 pathway , mTOR pathway and Wnt pathway. Phosphorylation and/or de-phosphorylation of p62-Ser349 may participate in the regulation of both selective autophagy and oxidative stress response (PMID: 33397898).