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  • Western Blot (WB) analysis of various lysates using FAK Recombinant antibody (81334-5-RR)

    WB analysis using 81334-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81334-5-RR (FAK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using FAK Recombinant antibody (81334-5-RR)

    IF Staining of MCF-7 using 81334-5-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using FAK antibody (81334-5-RR, Clone: 242295E4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using FAK Recombinant antibody (81334-5-RR)

    IF Staining of A549 using 81334-5-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using FAK antibody (81334-5-RR, Clone: 242295E4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 81334-5-RR

    Affinity and Kinetic Characterization of 81334-5-RR

    Biolayer interferometry (BLl) kinetic assays of 81334-5-RR against Human FAK were performed. The affinity constant is 2.03 nM.

FAK Recombinant antibody

FAK Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 81334-5-RR
Clone No.242295E4

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

PTK2, EC:2.7.10.2, FADK 1, FAK1, Focal adhesion kinase 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inA431 cells, HEK-293 cells, HeLa cells, MCF-7 cells, NIH/3T3 cells
Positive IF/ICC detected inA549 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81334-5-RR targets FAK in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FAK fusion protein Ag3331 Predict reactive species
Full Name PTK2 protein tyrosine kinase 2
Calculated Molecular Weight 1052 aa, 119 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC028733
Gene Symbol FAK
Gene ID (NCBI) 5747
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ05397
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971).

Protocols

Product Specific Protocols
WB protocol for FAK antibody 81334-5-RRDownload protocol
IF protocol for FAK antibody 81334-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols