FAK Recombinant antibody, PBS Only

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  WB analysis using 81334-5-RR (same clone as 81334-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81334-5-RR (FAK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81334-5-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 81334-5-RR (same clone as 81334-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using FAK antibody (81334-5-RR, Clone: 242295E4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81334-5-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 81334-5-RR (same clone as 81334-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using FAK antibody (81334-5-RR, Clone: 242295E4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81334-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81334-5-RR

  Biolayer interferometry (BLl) kinetic assays of 81334-5-RR against Human FAK were performed. The affinity constant is 2.03 nM.

FAK Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 81334-5-PBS

Clone No.242295E4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

PTK2, EC:2.7.10.2, FADK 1, FAK1, Focal adhesion kinase 1

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Product Information

81334-5-PBS targets FAK in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FAK fusion protein Ag3331
• Full Name: PTK2 protein tyrosine kinase 2
• Calculated Molecular Weight: 1052 aa, 119 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC028733
• Gene Symbol: FAK
• Gene ID (NCBI): 5747
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q05397
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971).