Tested Applications
| Positive WB detected in | MDA-MB-231 cells, HepG2 cells, HeLa cells, LNCaP cells, Jurkat cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human liver tissue |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
81538-1-RR targets VDAC1/Porin in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | voltage-dependent anion channel 1 |
| Calculated Molecular Weight | 31 kDa |
| Observed Molecular Weight | 31 kDa |
| GenBank Accession Number | NM_003374 |
| Gene Symbol | Porin |
| Gene ID (NCBI) | 7416 |
| RRID | AB_2935560 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P21796 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904). This antibody is specific to VDAC1.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for VDAC1/Porin antibody 81538-1-RR | Download protocol |
| IF protocol for VDAC1/Porin antibody 81538-1-RR | Download protocol |
| IHC protocol for VDAC1/Porin antibody 81538-1-RR | Download protocol |
| WB protocol for VDAC1/Porin antibody 81538-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
VDAC1/Porin Recombinant monoclonal antibody (2F4), catalog number 81538-1-RR, has 20 citations across 19 journals including J Nanobiotechnology, Advanced Science, Cell Death & Disease, Apoptosis, Plant Physiology, and International Immunopharmacology. Research fields cover mitochondrial biology, ferroptosis, cancer, myocardial ischemia-reperfusion injury, neurodegeneration, plant immunity, lipid metabolism, oxidative stress, and cell death pathways (apoptosis, PANoptosis, cuproptosis). Cited applications include WB, IF, and IP across human, mouse, and rat species.
| Species | Application | Title |
|---|---|---|
J Nanobiotechnology hUCMSC-exosomes attenuate acute lung injury by inhibiting ferroptosis in pulmonary microvascular endothelial cells through ribosomal protein RPS11 upregulation. | ||
Adv Sci (Weinh) Homoisoflavanone Delays Colorectal Cancer Progression via DNA Damage-Induced Mitochondrial Apoptosis and Parthanatos-Like Cell Death. | ||
Cell Death Dis Stattic enhances the anti-tumor activity of AZD4547 in LUSC by blocking STAT3/RRM2-mediated DNA repair and inducing ROS-driven mitochondrial dysfunction.
| ||
Cell Mol Biol Lett High-fat diet leads to male reproductive dysfunction by disrupting lipid-droplet-mediated organelle crosstalk. | ||
Apoptosis Acute high-altitude hypoxia induced NLRP3 inflammasome activation in pulmonary artery smooth muscle cells by BMAL1 targeting mitochondrial VDAC1-mediated MtDNA leakage | ||
Int J Nanomedicine Mitochondria-Rich Microvesicles Alleviate CNI ED by Transferring Mitochondria and Suppressing Local Ferroptosis |
Reviews
What customers say
Both reviewers used the antibody for Western Blot and reported strong performance. One user, working with a 231 cell line at 1:500 dilution, praised the product for producing a clear band with reduced or no nonspecific bands. The other, using HEK293 cells at 1:1000, found it effective as a mitochondrial marker control in organelle isolation, noting high signal with HRP secondaries after overnight 4°C incubation in 1% BSA. Overall, customers rate the antibody highly for specificity and signal strength in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Vignesh (Verified Customer) (09-03-2025) | It's an excellent product with reduced or no nonspecific bands and clear band.
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Monifa (Verified Customer) (08-31-2025) | The VDAC antibody worked well as a control for mitochondrial marker in organelle isolation. I used 1% BSA overnight in 4C. I visualized with HRP secondaries and signal is high. Will try higher dilutions like 1:5000 as recommended and for shorter time at room temperature to speed up the completion of my blots.
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