Tested Applications
| Positive WB detected in | MMS treated PC-3 cells, UV treated HeLa cells |
| Positive FC (Intra) detected in | MMS treated PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:10000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
81740-1-RR targets Phospho-CHEK2 (Thr68) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | CHK2 checkpoint homolog (S. pombe) |
| Calculated Molecular Weight | 61 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC004207 |
| Gene Symbol | CHEK2 |
| Gene ID (NCBI) | 11200 |
| RRID | AB_2935575 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O96017 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Serine/threonine-protein kinase Chk2 (CHEK2) is a serine/threonine kinase which is activated upon DNA damage and is implicated in pathways that govern DNA repair, cell cycle arrest or apoptosis in response to the initial damage. ATM phosphorylates CHEK2 on T68. Phosphorylation on T68 and subsequent full activation of CHEK2 was shown to require priming phosphorylation on adjacent residues by Polo-like kinase 3 (PLK3) and the dualspecificity tyrosine and serine/threoninekinase TTK/hMPS1. Additionally TTK appears to phosphorylate T68. Phosphorylation of T68 promotes the binding of the N-terminal SQ/TQ-rich cluster of one CHEK2 molecule with the FHA domain of another CHEK2 molecule. (PMID: 28553140, PMID: 18004398, PMID: 33322746)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-CHEK2 (Thr68) antibody 81740-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Phospho-CHEK2 (Thr68) Recombinant monoclonal antibody (Cat# 81740-1-RR) has 5 citations. They appear in Advanced Science, Journal of Ethnopharmacology, Biochemical Pharmacology, EMBO Reports, and Molecular Oncology. The research spans DNA damage response, intervertebral disc degeneration, PANoptosis in endometrial cancer, chemoresistance and homologous recombination repair, and replication stress in triple-negative breast cancer, with most studies using Western blot in human samples.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Elevated TRIM25 Impairs Poly (ADP-ribose) Metabolism via PARG Degradation and Mediates Compression-Induced Intervertebral Disc Degeneration. | ||
J Ethnopharmacol Chinese herbal prescription JZ-1 enhances host resistance to herpes simplex virus type 2 through regulating the MRN-ATM-CHK2 DNA damage response. | ||
Biochem Pharmacol Bavachinin exerts anti-tumor effects by activating TLR4/STING axis-dependent PANoptosis and synergistically enhances chemosensitivity in endometrial cancer | ||
EMBO Rep SPIN1 facilitates chemoresistance and HR repair by promoting Tip60 binding to H3K9me3 | ||
Mol Oncol Olaparib synergy screen reveals Exemestane induces replication stress in triple-negative breast cancer |
Reviews
What customers say
The single review for this product is highly positive, with a 5-star rating. The reviewer used the antibody at a 1:1000 dilution for Western Blot on MDA-MB-231 cell line lysates and reported an excellent experience, noting that it produced sharp, clear bands. Overall, the feedback highlights reliable performance and high specificity for WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Md Asad (Verified Customer) (01-20-2026) | Was excellent experience to get sharp bands in my western blot experiment on lysates prepared from MDA-MB-231 cell lines.
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