Tested Applications
| Positive WB detected in | RAW 264.7 cells |
| Positive IF-P detected in | human lung cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82191-3-RR targets IL-10 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg0026 Product name: Recombinant Mouse IL-10 protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec Tag: N-6*his Domain: 19-178 aa of NM_010548 Sequence: SRGQYSREDNNCTHFPVGQSHMLLELRTAFSQVKTFFQTKDQLDNILLTDSLMQDFKGYLGCQALSEMIQFYLVEVMPQAEKHGPEIKEHLNSLGEKLKTLRMRLRRCHRFLPCENKSKAVEQVKSDFNKLQDQGVYKAMNEFDIFINCIEAYMMIKMKS Predict reactive species |
| Full Name | interleukin 10 |
| Calculated Molecular Weight | 21 kDa |
| Observed Molecular Weight | 20 kDa |
| GenBank Accession Number | NM_010548 |
| Gene Symbol | Il10 |
| Gene ID (NCBI) | 16153 |
| RRID | AB_3670518 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P18893 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IL-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IL-10 antibody 82191-3-RR | Download protocol |
| WB protocol for IL-10 antibody 82191-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IL-10 Recombinant monoclonal antibody (Cat# 82191-3-RR) has accumulated 53 citations published in high-impact journals including Nature Communications, Advanced Materials, ACS Nano, Frontiers in Immunology, Journal of Ethnopharmacology, International Journal of Molecular Sciences, Acta Biomaterialia, and European Journal of Immunology. Associated research fields span diabetic wound healing, macrophage polarization and immunomodulation, neuroinflammation, bone and cartilage regeneration, atherosclerosis, liver and lung injury, intestinal inflammation, stem cell therapy, tissue engineering, and nanomedicine-based drug delivery, with applications primarily in mouse and rat models using WB, IHC, IF, and ELISA.
| Species | Application | Title |
|---|---|---|
Adv Mater Multifunctional Injectable Hydrogel as a Biomimic Lens: Optical-Mechanical Restoration and Dynamic Postoperative Microenvironment Regulation. | ||
Nat Commun Near-infrared light-driven nanomotors-based microneedles for the active therapy of bacterial infected acne. | ||
ACS Nano Single-Atom Nanozyme Driven Lactate Reversal Fuels Oxidative Metabolism and Represses Lactylation to Heal Diabetic Wounds. | ||
J Nanobiotechnology High glucose-primed HUVEC-derived extracellular vesicles encapsulated in microgels boost diabetic ischaemic flap regeneration via HIF-1α/VEGF pathway. | ||
NPJ Aging Time-restricted feeding improves functional capacity of adipose-derived stem cells with activation of OSK-associated transcriptional programs. | ||
Phytomedicine Huanglian Wendan ameliorates depression via gut-brain axis-related PPAR-α/allo pathways. |
