Tested Applications
| Positive WB detected in | rat brain tissue, Jurkat cells |
| Positive IHC detected in | mouse brain tissue, rat dorsal root ganglion tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82848-3-RR targets CHRNA7 (48 kDa) in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag15980 Product name: Recombinant human CHRNA7 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 130-321 aa of BC037571 Sequence: TVIVLQYHHHDPDGGKMPKWTRVILLNWCAWFLRMKRPGEDKVRPACQHKQRRCSLASVEMSAVAPPPASNGNLLYIGFRGLDGVHCVPTPDSGVVCGRMACSPTHDEHLLHGGQPPEGDPDLAKILEEVRYIANRFRCQDESEAVCSEWKFAACVVDRLCLMAFSVFTIICTIGILMSAPNFVEAVSKDFA Predict reactive species |
| Full Name | cholinergic receptor, nicotinic, alpha 7 |
| Calculated Molecular Weight | 502 aa, 56 kDa |
| Observed Molecular Weight | ~50 kDa |
| GenBank Accession Number | BC037571 |
| Gene Symbol | CHRNA7 |
| Gene ID (NCBI) | 1139 |
| RRID | AB_3670574 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P36544 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Nicotinic acetylcholine receptors (nAChRs) are cholinergic receptors that form ligand-gated ion channels that mediate fast signal transmission at synapses. CHRNA7 (neuronal acetylcholine receptor subunit alpha-7) forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. CHRNA7, so that like CHRNA7, the epithelial CHRFAM7A ORF encodes a protein (48 kDa) that differs from the CHRNA7 (58 kDa) by a distinct amino terminus and molecular weight.(PMID: 25473097)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CHRNA7 (48 kDa) antibody 82848-3-RR | Download protocol |
| WB protocol for CHRNA7 (48 kDa) antibody 82848-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CHRNA7 (48 kDa) Recombinant monoclonal antibody (2A10), catalog number 82848-3-RR, has 4 total citations. These appear in Free Radical Biology and Medicine, Frontiers in Immunology, Chemical Research in Toxicology, and Frontiers in Pharmacology. The associated research fields span diabetic wound healing and macrophage polarization, Hirschsprung-associated enterocolitis, nicotine-promoted lung cancer progression, and Alzheimer-like neuropathology. Applications cited include Western blot and immunofluorescence in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Free Radic Biol Med Ultrasound-Responsive Taurine Lipid Nanoparticles Attenuate Oxidative Stress and Promote Macrophage Polarization for Diabetic Wound Healing | ||
Front Immunol Acetylcholine from tuft cells promotes M2 macrophages polarization in Hirschsprung-associated enterocolitis | ||
Chem Res Toxicol The Role of HGF/c-Met/PI3K/AKT Pathway Regulated by α7-nAChR/β2-AR in Nicotine-Promoted Malignant Progression of Nonsmall Cell Lung Cancer A549 Cells. | ||
Front Pharmacol Adult-onset hippocampal α7 nicotinic acetylcholine receptor loss rapidly drives Alzheimer-like neuropathology in mice. |
