Tested Applications
| Positive WB detected in | MCF-7 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:150-1:600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82889-2-RR targets RBM17 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag0985 Product name: Recombinant human RBM17 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-308 aa of BC007871 Sequence: MSLYDDLGVETSDSKTEGWSKNFKLLQSQLQVKKAALTQAKSQRTKQSTVLAPVIDLKRGGSSDDRQIVDTPPHVAAGLKDPVPSGFSAGEVLIPLADEYDPMFPNDYEKVVKRQREERQRQRELERQKEIEEREKRRKDRHEASGFARRPDPDSDEDEDYERERRKRSMGGAAIAPPTSLVEKDKELPRDFPYEEDSRPRSQSSKAAIPPPVYEEQDRPRSPTGPSNSFLANMGGTVAHKIMQKYGFREGQGLGKHEQGLSTALSVEKTSKRGGKIIVGDATEKDASKKSDSNPLTEILKCPTKVVL Predict reactive species |
| Full Name | RNA binding motif protein 17 |
| Calculated Molecular Weight | 45 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | BC007871 |
| Gene Symbol | RBM17 |
| Gene ID (NCBI) | 84991 |
| RRID | AB_3670612 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96I25 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RNA-binding motif protein 17 (RBM17), also named splicing factor 45, was first identified as a member of the spliceosome. RBM17 expression is low in normal tissues but is overexpressed in several forms of cancer, including breast, ovarian and prostate.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RBM17 antibody 82889-2-RR | Download protocol |
| IHC protocol for RBM17 antibody 82889-2-RR | Download protocol |
| IP protocol for RBM17 antibody 82889-2-RR | Download protocol |
| WB protocol for RBM17 antibody 82889-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
