CoraLite® Plus 488-conjugated RBM17 Recombinant monoclonal antibody

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  IF Staining of HepG2 using CL488-82889-2

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 RBM17 antibody (CL488-82889-2, Clone: 230125A10 ) at dilution of 1:200, CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

RBM17 Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-82889-2

Clone No.230125A10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

230125A10, 45 kDa-splicing factor, RBM 17, RNA-binding motif protein 17, SPF 45

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82889-2 targets RBM17 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag0985

  Product name: Recombinant human RBM17 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-308 aa of BC007871

  Sequence: MSLYDDLGVETSDSKTEGWSKNFKLLQSQLQVKKAALTQAKSQRTKQSTVLAPVIDLKRGGSSDDRQIVDTPPHVAAGLKDPVPSGFSAGEVLIPLADEYDPMFPNDYEKVVKRQREERQRQRELERQKEIEEREKRRKDRHEASGFARRPDPDSDEDEDYERERRKRSMGGAAIAPPTSLVEKDKELPRDFPYEEDSRPRSQSSKAAIPPPVYEEQDRPRSPTGPSNSFLANMGGTVAHKIMQKYGFREGQGLGKHEQGLSTALSVEKTSKRGGKIIVGDATEKDASKKSDSNPLTEILKCPTKVVL

• Full Name: RNA binding motif protein 17
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC007871
• Gene Symbol: RBM17
• Gene ID (NCBI): 84991
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96I25
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RNA-binding motif protein 17 (RBM17), also named splicing factor 45, was first identified as a member of the spliceosome. RBM17 expression is low in normal tissues but is overexpressed in several forms of cancer, including breast, ovarian and prostate.