Tested Applications
| Positive WB detected in | A549 cells, HepG2 cells, L02 cells, HuH-7 cells, PC-3 cells |
| Positive IHC detected in | rat cerebellum tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat brain tissue |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:175-1:700 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:150-1:600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82930-1-RR targets RORA in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag0944 Product name: Recombinant human RORA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 223-523 aa of BC008831 Sequence: LDIQPSPDQSGLDINGIKPEPICDYTPASGFFPYCSFTNGETSPTVSMAELEHLAQNISKSHLETCQYLREELQQITWQTFLQEEIENYQNKQREVMWQLCAIKITEAIQYVVEFAKRIDGFMELCQNDQIVLLKAGSLEVVFIRMCRAFDSQNNTVYFDGKYASPDVFKSLGCEDFISFVFEFGKSLCSMHLTEDEIALFSAFVLMSADRSWLQEKVKIEKLQQKIQLALQHVLQKNHREDGILTKLICKVSTLRALCGRHTEKLMAFKAIYPDIVRLHFPPLYKELFTSEFEPAMQIDG Predict reactive species |
| Full Name | RAR-related orphan receptor A |
| Calculated Molecular Weight | 59 kDa |
| Observed Molecular Weight | 60-66 kDa |
| GenBank Accession Number | BC008831 |
| Gene Symbol | RORA |
| Gene ID (NCBI) | 6095 |
| RRID | AB_3670668 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P35398 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RORA is a steroid hormone receptor and has been implicated in the pathology of circadian rhythms, bone growth, angiogenesis, development of cones, cellular metabolism and a mediator in the immune and lipid metabolism pathways [PMID:21998696]. It binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence, which binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPAR gamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM and ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1 [PMID:11053433,14570920].
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for RORA antibody 82930-1-RR | Download protocol |
| IF protocol for RORA antibody 82930-1-RR | Download protocol |
| IHC protocol for RORA antibody 82930-1-RR | Download protocol |
| WB protocol for RORA antibody 82930-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-RORα recombinant rabbit monoclonal antibody (Cat# 82930-1-RR) has 10 citations. These appear in journals including Phytomedicine, Aging Cell, Frontiers in Immunology, Journal of Ethnopharmacology, Antioxidants & Redox Signaling, Communications Biology, BMC Genomics, Frontiers in Medicine, Current Medicinal Chemistry, and PLoS One. Research fields span circadian rhythm regulation, cataract and lens biology, lung cancer immunotherapy, asthma inflammation, hepatic lipid metabolism, myopia, pulmonary fibrosis, and gastric cancer.
| Species | Application | Title |
|---|---|---|
Phytomedicine Ji-Ming-San enhances intestinal circadian rhythms and mitigates colitis in mice: The role of epithelial RORα | ||
Aging Cell RORA Targeting PRNP Modulates Age-Related Cataract via Activation Oxidative Injury-Induced Cellular Senescence and Apoptosis of Lens Epithelial Cells. | ||
Front Immunol Administration of statins is correlated with favourable prognosis in lung cancer patients receiving immune checkpoint inhibitors.
| ||
J Ethnopharmacol Shuangpi formula treats neutrophilic asthma via circadian-mediated anti-inflammatory mechanisms. | ||
Antioxid Redox Signal Targeting Retinoic Acid Receptor-Related Orphan Receptor α to Prevent Cataracts: A New Pathway Through Nerve Growth Factor Activation. | ||
Commun Biol HlncRNA-1 integrates the hepatic circadian clock with lipid metabolism by targeting Hdlbp. |
