RORA Recombinant antibody, PBS Only

RORA Recombinant Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230217G6

Cat no : 82930-1-PBS

Synonyms

DKFZp686M2414, NR1F1, Nuclear receptor ROR alpha, Nuclear receptor RZR alpha, RAR related orphan receptor A, ROR1, ROR2, ROR3, RORA, RZR ALPHA, RZRA

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Validation Data Gallery

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  WB analysis using 82930-1-RR (same clone as 82930-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82930-1-RR (RORA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82930-1-PBS in a different storage buffer formulation.

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  IHC staining of rat cerebellum using 82930-1-RR (same clone as 82930-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 82930-1-RR (RORA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82930-1-PBS in a different storage buffer formulation.

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  IHC staining of rat cerebellum using 82930-1-RR (same clone as 82930-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 82930-1-RR (RORA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82930-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82930-1-RR (same clone as 82930-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RORA antibody (82930-1-RR, Clone: 230217G6 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82930-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82930-1-RR (same clone as 82930-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug RORA Recombinant antibody (82930-1-RR, Clone:230217G6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82930-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82930-1-PBS targets RORA in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RORA fusion protein Ag0944
• Full Name: RAR-related orphan receptor A
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 60-66 kDa
• GenBank Accession Number: BC008831
• Gene Symbol: RORA
• Gene ID (NCBI): 6095
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

RORA is a steroid hormone receptor and has been implicated in the pathology of circadian rhythms, bone growth, angiogenesis, development of cones, cellular metabolism and a mediator in the immune and lipid metabolism pathways [PMID:21998696]. It binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence, which binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPAR gamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM and ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1 [PMID:11053433,14570920].