Tested Applications
| Positive WB detected in | Cobalt Chloride treated NIH/3T3 cells |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82989-4-RR targets Hif-1 alpha in WB, IHC, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag33620 Product name: Recombinant mouse Hif1a protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 530-825 aa of NM_001313919 Sequence: SEYCFDVDSDMVNVFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESNSPSPPSMSTVTGFQQTQLQKPTITATATTTATTDESKTETKDNKEDIKILIASPSSTQVPQETTTAKASAYSGTHSRTASPDRAGKRVIEQTDKAHPRSLNLSATLNQRNTVPEEELNPKTIASQNAQRKRKMEHDGSLFQAAGIGTLLQQPGDCAPTMSLSWKRVKGFISSEQNGTEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQG* Predict reactive species |
| Full Name | hypoxia inducible factor 1, alpha subunit |
| Calculated Molecular Weight | 93 kDa |
| Observed Molecular Weight | 120 kDa |
| GenBank Accession Number | NM_001313919 |
| Gene Symbol | Hif1a |
| Gene ID (NCBI) | 15251 |
| RRID | AB_3670737 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q61221 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Hif-1 alpha antibody 82989-4-RR | Download protocol |
| WB protocol for Hif-1 alpha antibody 82989-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Hif-1 alpha Recombinant monoclonal antibody (230101E8), catalog number 82989-4-RR, has 16 citations published in journals including Advanced Science, Biomaterials, Carbohydrate Polymers, Phytomedicine, Materials Today Bio, Advanced Healthcare Materials, Acta Biomaterialia, ACS Applied Materials & Interfaces, International Immunopharmacology, PLoS One, Histology and Histopathology, European Journal of Histochemistry, and International Immunology. Associated research fields encompass tumor immunotherapy, diabetic wound healing, osteoarthritis, liver fibrosis, neuroinflammation, ulcerative colitis, and cancer biology.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) FSTL1 Orchestrates Metabolic-Epigenetic Crosstalk: Glycolysis-Dependent H3K18 Lactylation Drives Cartilage Fibrosis in Osteoarthritis. | ||
Biomaterials Engineered bacterial outer membrane vesicles enhanced tumor immunotherapy through remodeling tumor stroma and targeted delivery of CD73 siRNA. | ||
Carbohydr Polym Carboxymethyl chitosan and sodium alginate oxide pH-sensitive dual-release hydrogel for diabetes wound healing: The combination of astilbin liposomes and diclofenac sodium | ||
Phytomedicine Modulation of gut microbiota by the herbal formulation Intestinal Flora Balance rescues pancreatic and intestinal microvascular dysfunction to alleviate type 2 diabetes mellitus. | ||
Mater Today Bio Immediate tumor killing and long-term anti-tumor immunoreaction induced by Bufalin-loaded phototherapeutic Janus membrane in CRC postoperative therapy. | ||
Mater Today Bio Mitochondria-targeted cuproptosis-driven nanoplatform for synergistic photothermal/chemodynamic therapy and systemic antitumor immunotherapy. |
