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Endoglin/CD105 Recombinant monoclonal antibody (82993-6-RR)

Endoglin/CD105 Recombinant monoclonal antibody

Endoglin/CD105 Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 82993-6-RR

Clone No. 241196G9

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Synonyms

CD105, 241196G9, END, Endoglin, ENG

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

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Filter: AllWBIHC-AutostainerIHCIF-P

WB analysis using 82993-6-RR
Various lysates were subjected to SDS PAGE followed by western blot with 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 82993-6-RR
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colorectal cancer using 82993-6-RR
Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human pancreas cancer using 82993-6-RR
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary cancer using 82993-6-RR
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human tonsillitis using 82993-6-RR
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 82993-6-RR
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human appendicitis using 82993-6-RR
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 82993-6-RR
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary cancer using 82993-6-RR
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 82993-6-RR
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human tonsillitis using 82993-6-RR
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human tonsillitis using 82993-6-RR
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 82993-6-RR
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary cancer using 82993-6-RR
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colorectal cancer using 82993-6-RR
Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human pancreas cancer using 82993-6-RR
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 82993-6-RR
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of human tonsillitis using 82993-6-RR
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using Endoglin/CD105 antibody (82993-6-RR, Clone: 241196G9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Endoglin/CD105 antibody (67075-1-Ig, Clone: 2D5E8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of human tonsillitis using 82993-6-RR
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using Endoglin/CD105 antibody (82993-6-RR, Clone: 241196G9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Endoglin/CD105 antibody (67075-1-Ig, Clone: 2D5E8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for Endoglin/CD105 in ?

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, HUVEC cells, JAR cells
Positive IHC detected in	human ovary cancer tissue, human liver tissue, human liver cancer tissue, human tonsillitis tissue, human pancreas cancer tissue, human colorectal cancer tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human tonsillitis tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)-P	IF-P : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82993-6-RR targets Endoglin/CD105 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg0587 Product name: Recombinant Human Endoglin/CD105 protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6HIS Tag: C-6HIS Domain: 26-586 aa of BC014271 Sequence: ETVHCDLQPVGPERGEVTYTTSQVSKGCVAQAPNAILEVHVLFLEFPTGPSQLELTLQASKQNGTWPREVLLVLSVNSSVFLHLQALGIPLHLAYNSSLVTFQEPPGVNTTELPSFPKTQILEWAAERGPITSAAELNDPQSILLRLGQAQGSLSFCMLEASQDMGRTLEWRPRTPALVRGCHLEGVAGHKEAHILRVLPGHSAGPRTVTVKVELSCAPGDLDAVLILQGPPYVSWLIDANHNMQIWTTGEYSFKIFPEKNIRGFKLPDTPQGLLGEARMLNASIVASFVELPLASIVSLHASSCGGRLQTSPAPIQTTPPKDTCSPELLMSLIQTKCADDAMTLVLKKELVAHLKCTITGLTFWDPSCEAEDRGDKFVLRSAYSSCGMQVSASMISNEAVVNILSSSSPQRKKVHCLNMDSLSFQLGLYLSPHFLQASNTIEPGQQSFVQVRVSPSVSEFLLQLDSCHLDLGPEGGTVELIQGRAAKGNCVSLLSPSPEGDPRFSFLLHFYTVPIPKTGTLSCTVALRPKTGSQDQEVHRTVFMRLNIISPDLSGCTSKG Predict reactive species
Full Name	endoglin
Calculated Molecular Weight	71 kDa
Observed Molecular Weight	75-90 kDa
GenBank Accession Number	BC014271
Gene Symbol	CD105
Gene ID (NCBI)	2022
RRID	AB_3670742
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P17813
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

Protocols

Product Specific Protocols
IF protocol for Endoglin/CD105 antibody 82993-6-RR	Download protocol
IHC protocol for Endoglin/CD105 antibody 82993-6-RR	Download protocol
WB protocol for Endoglin/CD105 antibody 82993-6-RR	Download protocol