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Endoglin/CD105 Recombinant monoclonal antibody, PBS Only (82993-6-PBS)

Endoglin/CD105 Recombinant monoclonal antibody, PBS Only

Name: Endoglin/CD105 Recombinant monoclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 82993-6-PBS-100UG

Endoglin/CD105 Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 82993-6-PBS

Clone No. 241196G9

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

CD105, 241196G9, END, Endoglin, ENG

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

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Filter: AllWBIHC-AutostainerIHCIF-P

WB analysis using 82993-6-RR (same clone as 82993-6-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82993-6-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 82993-6-RR (same clone as 82993-6-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 82993-6-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colorectal cancer using 82993-6-RR (same clone as 82993-6-PBS)
Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 82993-6-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human pancreas cancer using 82993-6-RR (same clone as 82993-6-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 82993-6-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary cancer using 82993-6-RR (same clone as 82993-6-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 82993-6-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human tonsillitis using 82993-6-RR (same clone as 82993-6-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 82993-6-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 82993-6-RR (same clone as 82993-6-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 82993-6-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human appendicitis using 82993-6-RR (same clone as 82993-6-PBS)
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 82993-6-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 82993-6-RR (same clone as 82993-6-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 82993-6-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary cancer using 82993-6-RR (same clone as 82993-6-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82993-6-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 82993-6-RR (same clone as 82993-6-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82993-6-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human tonsillitis using 82993-6-RR (same clone as 82993-6-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82993-6-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human tonsillitis using 82993-6-RR (same clone as 82993-6-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82993-6-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 82993-6-RR (same clone as 82993-6-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82993-6-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary cancer using 82993-6-RR (same clone as 82993-6-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82993-6-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colorectal cancer using 82993-6-RR (same clone as 82993-6-PBS)
Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82993-6-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human pancreas cancer using 82993-6-RR (same clone as 82993-6-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82993-6-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 82993-6-RR (same clone as 82993-6-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82993-6-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of human tonsillitis using 82993-6-RR (same clone as 82993-6-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using Endoglin/CD105 antibody (82993-6-RR, Clone: 241196G9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Endoglin/CD105 antibody (67075-1-Ig, Clone: 2D5E8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82993-6-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using Endoglin/CD105 antibody (82993-6-RR, Clone: 241196G9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Endoglin/CD105 antibody (67075-1-Ig, Clone: 2D5E8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82993-6-PBS in a different storage buffer formulation.
IF Staining of human tonsillitis using 82993-6-RR (same clone as 82993-6-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using Endoglin/CD105 antibody (82993-6-RR, Clone: 241196G9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Endoglin/CD105 antibody (67075-1-Ig, Clone: 2D5E8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82993-6-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using Endoglin/CD105 antibody (82993-6-RR, Clone: 241196G9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Endoglin/CD105 antibody (67075-1-Ig, Clone: 2D5E8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82993-6-PBS in a different storage buffer formulation.

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Tested Applications
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Product Information

82993-6-PBS targets Endoglin/CD105 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg0587 Product name: Recombinant Human Endoglin/CD105 protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6HIS Tag: C-6HIS Domain: 26-586 aa of BC014271 Sequence: ETVHCDLQPVGPERGEVTYTTSQVSKGCVAQAPNAILEVHVLFLEFPTGPSQLELTLQASKQNGTWPREVLLVLSVNSSVFLHLQALGIPLHLAYNSSLVTFQEPPGVNTTELPSFPKTQILEWAAERGPITSAAELNDPQSILLRLGQAQGSLSFCMLEASQDMGRTLEWRPRTPALVRGCHLEGVAGHKEAHILRVLPGHSAGPRTVTVKVELSCAPGDLDAVLILQGPPYVSWLIDANHNMQIWTTGEYSFKIFPEKNIRGFKLPDTPQGLLGEARMLNASIVASFVELPLASIVSLHASSCGGRLQTSPAPIQTTPPKDTCSPELLMSLIQTKCADDAMTLVLKKELVAHLKCTITGLTFWDPSCEAEDRGDKFVLRSAYSSCGMQVSASMISNEAVVNILSSSSPQRKKVHCLNMDSLSFQLGLYLSPHFLQASNTIEPGQQSFVQVRVSPSVSEFLLQLDSCHLDLGPEGGTVELIQGRAAKGNCVSLLSPSPEGDPRFSFLLHFYTVPIPKTGTLSCTVALRPKTGSQDQEVHRTVFMRLNIISPDLSGCTSKG Predict reactive species
Full Name	endoglin
Calculated Molecular Weight	71 kDa
Observed Molecular Weight	75-90 kDa
GenBank Accession Number	BC014271
Gene Symbol	CD105
Gene ID (NCBI)	2022
RRID	AB_3670742
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P17813
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)