Tested Applications
| Positive WB detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82997-7-RR targets Phospho-FOXO1 (Ser319) in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | forkhead box O1 |
| Calculated Molecular Weight | 655 aa, 70 kDa |
| Observed Molecular Weight | 70-80 kDa |
| GenBank Accession Number | BC021981 |
| Gene Symbol | FOXO1 |
| Gene ID (NCBI) | 2308 |
| RRID | AB_3743451 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q12778 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The forkhead box class O (FoxO) family in mammals is comprised of the evolutionally highly conserved forkhead transcription factors, FoxO1, FoxO3a, FoxO4, and FoxO6. FoxO1 is highly expressed in lymphoid cells, especially in T and B cells. FoxO1 phosphorylation determines the transcriptional activity and cellular localization of FoxO1. Results showed that GLK-IKKβ signaling triggered the nuclear export of FoxO1. (PMID: 35966593)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-FOXO1 (Ser319) antibody 82997-7-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The product received a single 5-star review from a user who performed Western Blot on HEPG2 cells using a 1:500 dilution. No written comments were provided, but the top rating indicates strong satisfaction with the antibody's performance in this application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Vignesh (Verified Customer) (01-28-2026) |
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