Phospho-FOXO1 (Ser319) Recombinant monoclonal antibody, PBS Only

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  WB analysis using 82997-7-RR (same clone as 82997-7-PBS)

  HEK-293T and MCF-7 cell lysates were subjected to SDS PAGE followed by western blot with 82997-7-RR (Phospho-FOXO1 (Ser319) antibody) blocked with BSA only, Phospho-FOXO1 (Ser319) peptide or non-Phospho peptide at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 82997-7-PBS in a different storage buffer formulation.

Phospho-FOXO1 (Ser319) Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 82997-7-PBS

Clone No.250275C3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

FOXO1 (Ser319), p FOXO1 (Ser319), p-FOXO1, p-FOXO1 (Ser319), p-FOXO1(Ser319)

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Product Information

82997-7-PBS targets Phospho-FOXO1 (Ser319) in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: forkhead box O1
• Calculated Molecular Weight: 655 aa, 70 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC021981
• Gene Symbol: FOXO1
• Gene ID (NCBI): 2308
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q12778
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The forkhead box class O (FoxO) family in mammals is comprised of the evolutionally highly conserved forkhead transcription factors, FoxO1, FoxO3a, FoxO4, and FoxO6. FoxO1 is highly expressed in lymphoid cells, especially in T and B cells. FoxO1 phosphorylation determines the transcriptional activity and cellular localization of FoxO1. Results showed that GLK-IKKβ signaling triggered the nuclear export of FoxO1. (PMID: 35966593)