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KD/KO Validated

MTA3 Recombinant monoclonal antibody

MTA3 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 83042-3-RR

Clone No. 230350D12

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

230350D12, Metastasis-associated protein MTA3, MTA 3, mta1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 750

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Filter: AllWBIHCIF/ICCFC (Intra)Affinity

WB analysis using 83042-3-RR
Various lysates were subjected to SDS PAGE followed by western blot with 83042-3-RR (MTA3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of MCF-7 using 83042-3-RR
WB result of MTA3 antibody (83042-3-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTA3 transfected MCF-7 cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse spleen using 83042-3-RR
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83042-3-RR (MTA3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 83042-3-RR
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83042-3-RR (MTA3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human tonsillitis using 83042-3-RR
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83042-3-RR (MTA3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse colon using 83042-3-RR
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83042-3-RR (MTA3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse kidney using 83042-3-RR
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83042-3-RR (MTA3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat colon using 83042-3-RR
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 83042-3-RR (MTA3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat kidney using 83042-3-RR
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 83042-3-RR (MTA3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat spleen using 83042-3-RR
Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 83042-3-RR (MTA3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of U-251 using 83042-3-RR
Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MTA3 antibody (83042-3-RR, Clone: 230350D12 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of U-251 using 83042-3-RR
1x10^6 U-251 cells were intracellularly stained with 0.25 ug MTA3 Recombinant antibody (83042-3-RR, Clone:230350D12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity and Kinetic Characterization of 83042-3-RR
Biolayer interferometry (BLl) kinetic assays of 83042-3-RR against Human MTA3 were performed. The affinity constant is below 1 pM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for MTA3 in ?

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	Jurkat cells, MCF-7 cells, A375 cells, NIH/3T3 cells, C2C12 cells, Raji cells
Positive IHC detected in	rat spleen tissue, mouse spleen tissue, human placenta tissue, human tonsillitis tissue, mouse colon tissue, mouse kidney tissue, rat colon tissue, rat kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	U-251 cells
Positive FC (Intra) detected in	U-251 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83042-3-RR targets MTA3 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag6400 Product name: Recombinant human MTA3 protein Source: e coli.-derived, PKG Tag: GST Domain: 1-343 aa of BC053631 Sequence: MAANMYRVGDYVYFENSSSNPYLIRRIEELNKTASGNVEAKVVCFYRRRDISNTLIMLADKHAKEIEEESETTVEADLTDKQKHQLKHRELFLSRQYESLPATHIRGKCSVALLNETESVLSYLDKEDTFFYSLVYDPSLKTLLADKGEIRVGPRYQADIPEMLLEGESDEREQSKLEVKVWDPNSPLTDRQIDQFLVVARAVGTFARALDCSSSVRQPSLHMSAAAASRDITLFHAMDTLYRHSYDLSSAISVLVPLGGPVLCRDEMEEWSASEASLFEEALEKYGKDFNDIRQDFLPWKSLTSIIEYYYMWKTTDRYVQQKRLKAAEAESKLKQVYIPTYS Predict reactive species
Full Name	metastasis associated 1 family, member 3
Calculated Molecular Weight	68 kDa
Observed Molecular Weight	62 kDa, 68 kDa
GenBank Accession Number	BC053631
Gene Symbol	MTA3
Gene ID (NCBI)	57504
RRID	AB_3670772
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9BTC8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The metastasis-associated (MTA) family contains three members, MTA1, MTA2 and MTA3 (PMID:12167865).Metastasis-associated protein 3 (MTA3) is a cell-type specific component of the Mi-2-NURD transcriptional co-repressor complex. It has a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels.

Protocols

Product Specific Protocols
IF protocol for MTA3 antibody 83042-3-RR	Download protocol
IHC protocol for MTA3 antibody 83042-3-RR	Download protocol
WB protocol for MTA3 antibody 83042-3-RR	Download protocol