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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UFL1 Recombinant monoclonal antibody (83047-5-RR)

    IF Staining of HepG2 using 83047-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using UFL1 antibody (83047-5-RR, Clone: 230363G7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Flow cytometry (FC) experiment of U2OS cells using UFL1 Recombinant monoclonal antibody (83047-5-RR)

    FC experiment of U2OS using 83047-5-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human UFL1 (83047-5-RR, Clone:230363G7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 83047-5-RR

    Affinity and Kinetic Characterization of 83047-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83047-5-RR against Human KIAA0776 were performed. The affinity constant is below 1 pM.

UFL1 Recombinant monoclonal antibody

UFL1 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83047-5-RR
Clone No.230363G7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

KIAA0776, 230363G7, E3 UFM1 protein ligase 1, E3 UFM1-protein ligase 1, E3 UFM1-protein transferase 1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83047-5-RR targets UFL1 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag23362

Product name: Recombinant human KIAA0776 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC036379

Sequence: MADAWEEIRRLAADFQRAQFAEATQRLSERNCIEIVNKLIAQKQLEVVHTLDGKEYITPAQISKEMRDELHVRGGRVNIVDLQQVINVDLIHIENRIGDIIKSEKHVQLVLGQLIDENYLDRLAEEVNDKLQESGQVTISELCKTYDLPGNFLTQALTQRLGRIISGHIDLDNRGVIFTEAFVARHKARIRGLFSAITRPTAVNSLISKYGFQEQLLYSVLEELVNSGRLRGTVVGGRQDKAVFVPDIYSRTQSTWVDSFFRQNGYLEFDALSRLGIPDAVSYIKKRYKTTQLLFLKAACVGQGLVDQVEASVEEAISSGTWVDIAPLLPTSLSVEDAAILLQQVMRAFS

Predict reactive species
Full Name KIAA0776
GenBank Accession NumberBC036379
Gene Symbol KIAA0776
Gene ID (NCBI) 23376
RRIDAB_3670773
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO94874
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
FC protocol for UFL1 antibody 83047-5-RRDownload protocol
IF protocol for UFL1 antibody 83047-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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