Tested Applications
| Positive WB detected in | U266 cells, HeLa cells, MCF-7 cells |
| Positive IF/ICC detected in | U266 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83068-5-RR targets IFNAR2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg2928 Product name: Recombinant Human IFNAR2 protein (rFc Tag) (HPLC verified) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 27-243 aa of NM_207585.2 Sequence: ISYDSPDYTDESCTFKISLRNFRSILSWELKNHSIVPTHYTLLYTIMSKPEDLKVVKNCANTTRSFCDLTDEWRSTHEAYVTVLEGFSGNTTLFSCSHNFWLAIDMSFEPPEFEIVGFTNHINVMVKFPSIVEEELQFDLSLVIEEQSEGIVKKHKPEIKGNMSGNFTYIIDKLIPNTNYCVSVYLEHSDEQAVIKSPLKCTLLPPGQESESAESAK Predict reactive species |
| Full Name | interferon (alpha, beta and omega) receptor 2 |
| Calculated Molecular Weight | 58 kDa |
| Observed Molecular Weight | 90-100 kDa |
| GenBank Accession Number | NM_207585.2 |
| Gene Symbol | IFNAR2 |
| Gene ID (NCBI) | 3455 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P48551-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Interferon alpha/beta receptor 2 (IFNAR2) is a type I membrane protein that associates with IFNAR1 to form the receptor for type I interferons. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2, and then increase transcription of the IFN-induced genes whose products exert antiviral, immunomodulatory, and antiproliferative effects.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IFNAR2 antibody 83068-5-RR | Download protocol |
| WB protocol for IFNAR2 antibody 83068-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
