IFNAR2 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis of U266 using 83068-5-RR (same clone as 83068-5-PBS)

  U266 cells were subjected to SDS PAGE followed by western blot with 83068-5-RR (IFNAR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83068-5-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 83068-5-RR (same clone as 83068-5-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 83068-5-RR (IFNAR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83068-5-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 83068-5-RR (same clone as 83068-5-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 83068-5-RR (IFNAR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83068-5-PBS in a different storage buffer formulation.

• 

  IF Staining of U266 using 83068-5-RR (same clone as 83068-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U266 cells using IFNAR2 antibody (83068-5-RR, Clone: 250313D3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83068-5-PBS in a different storage buffer formulation.

IFNAR2 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 83068-5-PBS

Clone No.250313D3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

IFN alpha REC, IFN alpha/beta receptor 2, IFN R, IFN R 2, IFNABR

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Product Information

83068-5-PBS targets IFNAR2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2928

  Product name: Recombinant Human IFNAR2 protein (rFc Tag) (HPLC verified)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 27-243 aa of NM_207585.2

  Sequence: ISYDSPDYTDESCTFKISLRNFRSILSWELKNHSIVPTHYTLLYTIMSKPEDLKVVKNCANTTRSFCDLTDEWRSTHEAYVTVLEGFSGNTTLFSCSHNFWLAIDMSFEPPEFEIVGFTNHINVMVKFPSIVEEELQFDLSLVIEEQSEGIVKKHKPEIKGNMSGNFTYIIDKLIPNTNYCVSVYLEHSDEQAVIKSPLKCTLLPPGQESESAESAK

• Full Name: interferon (alpha, beta and omega) receptor 2
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: NM_207585.2
• Gene Symbol: IFNAR2
• Gene ID (NCBI): 3455
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P48551-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Interferon alpha/beta receptor 2 (IFNAR2) is a type I membrane protein that associates with IFNAR1 to form the receptor for type I interferons. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2, and then increase transcription of the IFN-induced genes whose products exert antiviral, immunomodulatory, and antiproliferative effects.