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  • Western Blot (WB) analysis of various lysates using RBM20 Recombinant antibody (83363-1-RR)

    WB analysis using 83363-1-RR (same clone as 83363-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83363-1-RR (RBM20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83363-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00379-4

    Sandwich ELISA standard curve of MP00379-4

    Sandwich ELISA standard curve of MP00379-4, Human RBM20 Recombinant Matched Antibody Pair - PBS only. 83363-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag34322. 83363-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Sample test of MP00379-4

    Sample test of MP00379-4

    The mean RBM20 concentration was determined to be 1.8 ng/mL in A549 cell extract based on a 2.0 mg/mL extract load.

  • Flow cytometry (FC) experiment of K-562 cells using RBM20 Recombinant antibody (83363-1-RR)

    FC experiment of K-562 using 83363-1-RR (same clone as 83363-1-PBS)

    1x10^6 K-562 cells were intracellularly stained with 0.2 ug RBM20 Recombinant antibody (83363-1-RR, Clone:240345B7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83363-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00379-1

    Cytometric bead array standard curve of MP00379-1

    Cytometric bead array standard curve of MP00379-1, RBM20 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83363-1-PBS. Detection antibody: 83363-2-PBS. Standard: Ag34322. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP00379-2

    Cytometric bead array standard curve of MP00379-2

    Cytometric bead array standard curve of MP00379-2, RBM20 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83363-3-PBS. Detection antibody: 83363-1-PBS. Standard: Ag34322. Range: 1.25-80 ng/mL.

  • Affinity and Kinetic Characterization of 83363-1-RR

    Affinity and Kinetic Characterization of 83363-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83363-1-RR against Human RBM20 were performed. The affinity constant is below 1 pM.

RBM20 Recombinant antibody, PBS Only (Capture/Detector)

RBM20 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83363-1-PBS
Clone No.240345B7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

RNA binding protein, RNA binding motif protein 20, 240345B7

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Product Information

83363-1-PBS targets RBM20 as part of a matched antibody pair:

MP00379-1: 83363-1-PBS capture and 83363-2-PBS detection (validated in Cytometric bead array)

MP00379-2: 83363-3-PBS capture and 83363-1-PBS detection (validated in Cytometric bead array)

MP00379-4: 83363-2-PBS capture and 83363-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RBM20 fusion protein Ag34322 Predict reactive species
Full Name RNA binding motif protein 20
Calculated Molecular Weight134 kDa
Observed Molecular Weight190 kDa
GenBank Accession NumberNM_001134363
Gene Symbol RBM20
Gene ID (NCBI) 282996
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ5T481
Storage Buffer PBS only, pH 7.3.
Storage ConditionsStore at -80°C.

Background Information

RBM20 (RNA binding motif protein 20) is a heart- and skeletal muscle- nriched splicing factor that controls tissue-specific isoform expression of many other genes besides TTN, including sarcomeric genes such myomesin 1, but also Ca2+- and ion-handling genes such as CAMK2D (calcium/calmodulin-dependent kinase II-delta) and RYR2 (ryanodine receptor 2) (PMID: 29650543).