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LIMS1 Recombinant monoclonal antibody (83368-3-RR)

LIMS1 Recombinant monoclonal antibody

LIMS1 Uni-rAb^® Recombinant Antibody for WB, IP, ELISA

Cat No. 83368-3-RR

Clone No.260013D6

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

LIM and senescent cell antigen-like-containing domain protein 1, Particularly interesting new Cys-His protein 1, PINCH, PINCH 1, PINCH1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

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WB analysis using 83368-3-RR
Various lysates were subjected to SDS PAGE followed by western blot with 83368-3-RR (LIMS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HeLa using 83368-3-RR
IP result of anti-LIMS1 (IP:83368-3-RR, 4ug; Detection:83368-3-RR 1:1000) with HeLa cells lysate 1000 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, A549 cells, Caco-2 cells, K-562 cells, Jurkat cells, mouse kidney tissue, rat kidney tissue
Positive IP detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83368-3-RR targets LIMS1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	LIM and senescent cell antigen-like domains 1
Calculated Molecular Weight	37 kDa
Observed Molecular Weight	35 kDa
GenBank Accession Number	NM_004987
Gene Symbol	LIMS1
Gene ID (NCBI)	3987
RRID	AB_3743477
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P48059
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LIMS1, also known as PINCH1, is an adaptor protein that contains five LIM domains, or double zinc fingers(PMID: 7517666). PINCH-1 is a widely expressed focal adhesion protein that forms a ternary complex with integrin-linked kinase (ILK) and CH-ILKBP/actopaxin/alpha-parvin (PMID: 14551191). LIMS1 is involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques.