LIMS1 Recombinant monoclonal antibody, PBS Only

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• LIMS1 Recombinant monoclonal antibody, PBS Only (83368-3-PBS)

LIMS1 Uni-rAb^® Recombinant Antibody for WB, IP, Indirect ELISA

Cat No. 83368-3-PBS

Clone No.260013D6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

LIM and senescent cell antigen-like-containing domain protein 1, Particularly interesting new Cys-His protein 1, PINCH, PINCH 1, PINCH1

• 

  WB analysis using 83368-3-RR (same clone as 83368-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83368-3-RR (LIMS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83368-3-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 83368-3-RR (same clone as 83368-3-PBS)

  IP result of anti-LIMS1 (IP:83368-3-RR, 4ug; Detection:83368-3-RR 1:1000) with HeLa cells lysate 1000 ug. This data was developed using the same antibody clone with 83368-3-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

83368-3-PBS targets LIMS1 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: LIM and senescent cell antigen-like domains 1
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: NM_004987
• Gene Symbol: LIMS1
• Gene ID (NCBI): 3987
• RRID: AB_3743477
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P48059
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

LIMS1, also known as PINCH1, is an adaptor protein that contains five LIM domains, or double zinc fingers(PMID: 7517666). PINCH-1 is a widely expressed focal adhesion protein that forms a ternary complex with integrin-linked kinase (ILK) and CH-ILKBP/actopaxin/alpha-parvin (PMID: 14551191). LIMS1 is involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques.